Histopathological Features of Gastrointestinal Stromal Tumors and the Contribution of DOG1 Expression to the Diagnosis
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Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: Gastrointestinal stromal tumors (GIST) have KIT or platelet-derived growth factor receptor ? (PDGFR?) mutations affecting receptor tyrosine ki-nase activity and do not benefit from classic treatment regimens.Aims: The aim of this study was to review the al-gorithm that may be followed for the diagnosis and differential diagnosis in GISTs by investigating the histomorphological parameters and expression charac-teristics of classical immunohistochemical antibodies used in routine tests in addition to DOG1 expression.Study Design: Diagnostic accuracy study.Methods: We reevaluated the histological and immu-nohistochemical parameters of 37 GISTs. The standard immunohistochemical diagnosis and differential diag-nosis panel antibodies (CD117, PDGFR?, CD34, vi-mentin, desmin, SMA, S-100, and Ki67) were studied on the tumor sections. We also used the popular marker DOG1 antibody with accepted sensitivity for GISTs in recent years and the PDGFR? immune marker for which the benefit in routine practice is discussed.Results: Classification according to progressive dis-ease risk groups of the 37 cases revealed that 54% were in the high risk, 19% in the moderate risk, 16% in the low risk, 8% in the very low risk and 8% in the no risk group.Cytological atypia, necrosis, mucosal invasion and the Ki67 index were found to be related to the progressive disease risk groups of the tumors (p<0.05).Positive immunoreaction was observed with CD117 and PDGFR? in all GISTs in the study (100%). Posi-tivity with the DOG1 antibody was found in 33 (89%) cases. CD34 was positive in 62% (23) of the cases.Conclusion: The CD117 antibody still plays a key role in GIST diagnosis. However, the use of DOG1 and PDGFR? antibodies combined with CD117 as sensitive markers can be beneficial
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Balkan Medical Journal
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
32
Sayı
4
