The effect of proanthocyanidin on formaldehyde-induced toxicity in rat testes
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background/aim: This study investigated the effect of proanthocyanidin (PA) against formaldehyde (FA)-induced lipid peroxidation damage and morphological changes in rat testes. Materials and methods: Twenty-one Wistar albino rats were randomized into 3 groups: control, FA, and FA + PA groups. Plasma and tissue malondialdehyde (MDA) and total sialic acid (TSA) levels were measured. Testes tissues were observed by light and electron microscopy. Results: TSA (plasma and tissue) levels decreased and MDA (plasma) significantly increased (P < 0.05) in rats treated with FA compared to the controls. Tissue MDA levels were not significantly different. Several necrotic changes were observed in testes tissues by light and electron microscopy. Disordering in epithelia of seminiferous tubules, vacuolization between germinal epithelium cells, and separated basement membranes were observed by light microscope. Immunopositivity in Leydig cells decreased in the FA group (P < 0.05). In the FA + PA group there were more immune Leydig cells reacting immune-positively than in the FA group (P < 0.05). Ultrastructurally, FA also caused disorganization and loss of mitochondrial cristae, and dilatation in endoplasmic reticulum in testes. Conclusion: The results suggest that PA has a protective effect on FA toxicity in testes.
Açıklama
Anahtar Kelimeler
Formaldehyde Toxicity, Malondialdehyde, Proanthocyanidin, Sialic Acid, Testis, Serum Sialic-Acid, Oxidative Damage, Vitamin-E, Seminiferous Tubules, Extracellular-Matrix, Procyanidin Extract, Lipid-Peroxidation, Grape, Expression, Melatonin
Kaynak
Turkish Journal Of Medical Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
46
Sayı
1
