A New Tube Gastrostomy Model in Animal Experiments
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Derman Medical Publ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: The orogastric route is the most preferred application method in the vast majority of the animal experiments in which application can be achieved by adding the material to the water of the experiment animal, through an orogastric tube or with a surgically managed ostomy. Material and Method: This experiment was constructed with twelve male Sprague-Dawley rats which were randomly assigned to one of two groups consist of control group (group C, n:6) and tube gastrostomy group (group TG, n:6). A novel and simple gastrostomy tube was derivated from a silicone foley catheter. Tube gastrostomy apparatus was constituted with a silicone foley catheter (6 French). In the group TG an incision was performed, and the stomach was visualized. A 1 cm incision was made in the midline and opening of the peritoneum. Anchoring sutures were placed and anterior gastric wall was lifted. The gastric wall is then opened. The apparatus was placed into the stomach and pulled through from a tunnel under the skin and fixed to the lateral abdominal wall with a 2/0 silk suture. Result: The procedure was ended in the 10th day of experiment. No mortality was observed in group C. The rats were monitored daily and no abnormal behavior consists of self harming incision site, resistance to oral intake or attending to displace. There was statistically significant difference in increasing alanine transaminase level (p<0.05) and decrease in the total protein and body weight (p<0.05) at the group TG at the end of experiment. There was significant increase in urea levels in Group C (p<0.05) at the end of experiment. The statistically significant decrease was observed in the same period in group C between aspartate transaminase, albumin, total protein, and body weight (p<0.05). Glucose (p=0.047) and aspartate transaminase (p=0.050) level decrease changes and weight loose (p=0.034) from preoperative period to the end of the experiment between gastrostomy and laparotomy groups were statistically significantly. Discussion: Here in we presented a novel and simple tube gastrostomy model in an experimental rat model in which rats had unrestrained movements. We believe that this new constructed tube gastrostomy model may be an alternative route for the experimental models in which orogastric route is unavailable to use.
Açıklama
Anahtar Kelimeler
Animal Experimentation, Experimental Designs, Gastrostomy
Kaynak
Journal Of Clinical And Analytical Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
4
Sayı
1
