Genotoxic Effects of Heavy Metal Mixture in Drosophila melanogaster: Expressions of Heat Shock Proteins, RAPD Profiles and Mitochondrial DNA Sequence

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorTabakcioglu, Kiymet
dc.date.accessioned2024-06-12T11:02:34Z
dc.date.available2024-06-12T11:02:34Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe genotoxic effects of four heavy metal mixtures on Drosophila melanogaster were investigated with reference to gene expressions of heat shock proteins (HSP26, HSP60, HSP70 and HSP83), DNA profiles, and mitochondrial NADH dehydrogenase sequence. Adult D. melanogaster flies were treated with a mixture of four (Fe, Cu, Cd and Pb) heavy metals (HMs) in three different concentrations, which were selected based on one higher dose (HM3) and one lower dose (HM1) relative to the permitted limits (HM2) in drinking water at 1st, 5th and 10th days. It was determined that the amount of the accumulated heavy metals and the expressions of the HSP genes were changed with increasing exposure time. The accumulations of Cd and Pb were increased with increasing exposure time; additionally, the HSP expression patterns were determined as HSP70>HSP60>HSP26>HSP83 HM1 (5th day), HM2 (5th day and 10th day), and HM3 (all exposure times). It was also determined that the application of the heavy metal mixture affected the random amplified polymorphic DNA (RAPD) profiles and the mitochondrial NADH dehydrogenase sequence of D. melanogaster. The highest base pair changes (9 bp) were determined at the HM2 concentration (permissible limits in drinking water) on the 1st day of treatment. Therefore, it was shown that mixture of four heavy metals caused a genotoxic effect and D. melanogaster is a useful model organism for heavy metal-induced genotoxicity studies.en_US
dc.description.sponsorshipTrakya University Research Project Foundation, Edirne,/Turkey [TUBAP-2013/118]; T.R. State Planning Organization [2011K120390]en_US
dc.description.sponsorshipThis study was supported by Trakya University Research Project Foundation (Project Number: TUBAP-2013/118), Edirne,/Turkey. The authors are grateful to Prof. Dr. Yener YORUK (Rector of Trakya University, Edirne,/Turkey) and the Technology Research and Application Centre (TUTAGEM), which is funded by T.R. State Planning Organization (Project Number: 2011K120390), for providing the laboratory equipment.en_US
dc.identifier.doi10.1007/s11270-014-2104-9
dc.identifier.issn0049-6979
dc.identifier.issn1573-2932
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-84905311663en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11270-014-2104-9
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21338
dc.identifier.volume225en_US
dc.identifier.wosWOS:000341836700024en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer International Publishing Agen_US
dc.relation.ispartofWater Air And Soil Pollutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenotoxicityen_US
dc.subjectDrinking Wateren_US
dc.subjectHeavy Metal Mixtureen_US
dc.subjectIronen_US
dc.subjectCadmiumen_US
dc.subjectLeaden_US
dc.subjectCopperen_US
dc.subjectHeat Shock Proteinsen_US
dc.subjectRAPDen_US
dc.subjectMtdnaen_US
dc.subjectAmplified Polymorphic Dnaen_US
dc.subjectIn-Vivo Genotoxicityen_US
dc.subjectOxidative Stressen_US
dc.subjectGene-Expressionen_US
dc.subjectMolecular Chaperonesen_US
dc.subjectFitness Parametersen_US
dc.subjectInduced Toxicityen_US
dc.subjectExposureen_US
dc.subjectHsp70en_US
dc.subjectInductionen_US
dc.titleGenotoxic Effects of Heavy Metal Mixture in Drosophila melanogaster: Expressions of Heat Shock Proteins, RAPD Profiles and Mitochondrial DNA Sequenceen_US
dc.typeArticleen_US

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