Genotoxic Effects of Heavy Metal Mixture in Drosophila melanogaster: Expressions of Heat Shock Proteins, RAPD Profiles and Mitochondrial DNA Sequence
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer International Publishing Ag
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The genotoxic effects of four heavy metal mixtures on Drosophila melanogaster were investigated with reference to gene expressions of heat shock proteins (HSP26, HSP60, HSP70 and HSP83), DNA profiles, and mitochondrial NADH dehydrogenase sequence. Adult D. melanogaster flies were treated with a mixture of four (Fe, Cu, Cd and Pb) heavy metals (HMs) in three different concentrations, which were selected based on one higher dose (HM3) and one lower dose (HM1) relative to the permitted limits (HM2) in drinking water at 1st, 5th and 10th days. It was determined that the amount of the accumulated heavy metals and the expressions of the HSP genes were changed with increasing exposure time. The accumulations of Cd and Pb were increased with increasing exposure time; additionally, the HSP expression patterns were determined as HSP70>HSP60>HSP26>HSP83 HM1 (5th day), HM2 (5th day and 10th day), and HM3 (all exposure times). It was also determined that the application of the heavy metal mixture affected the random amplified polymorphic DNA (RAPD) profiles and the mitochondrial NADH dehydrogenase sequence of D. melanogaster. The highest base pair changes (9 bp) were determined at the HM2 concentration (permissible limits in drinking water) on the 1st day of treatment. Therefore, it was shown that mixture of four heavy metals caused a genotoxic effect and D. melanogaster is a useful model organism for heavy metal-induced genotoxicity studies.
Açıklama
Anahtar Kelimeler
Genotoxicity, Drinking Water, Heavy Metal Mixture, Iron, Cadmium, Lead, Copper, Heat Shock Proteins, RAPD, Mtdna, Amplified Polymorphic Dna, In-Vivo Genotoxicity, Oxidative Stress, Gene-Expression, Molecular Chaperones, Fitness Parameters, Induced Toxicity, Exposure, Hsp70, Induction
Kaynak
Water Air And Soil Pollution
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
225
Sayı
9
