Inhibitory effect of curcuminoid pretreatments on endogenous dentin proteases

Basit Öğe Kaydı
dc.authoridATAY, MELTEM TEKBAS/0000-0002-1762-830X
dc.authoridTEKBAS ATAY, MELTEM/0000-0002-1762-830X
dc.authoridTezvergil-Mutluay, Arzu/0000-0003-0932-8531
dc.authorwosidATAY, MELTEM TEKBAS/F-1130-2018
dc.authorwosidTEKBAS ATAY, MELTEM/W-7451-2018
dc.authorwosidTezvergil-Mutluay, Arzu/B-4067-2008
dc.contributor.authorSeseogullari-Dirihan, Roda
dc.contributor.authorTekbas Atay, Meltem
dc.contributor.authorPashley, David Henry
dc.contributor.authorTezvergil-Mutluay, Arzu
dc.date.accessioned2024-06-12T10:59:12Z
dc.date.available2024-06-12T10:59:12Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 0/1 of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhIVIMP-9 were measured using an activity assay. Data were subjected to repeated-measures-ANOVA (alpha=0.05). Gelatinolytic activity was analyzed using zymography. ICTP and CTX release and dry mass loss of curcuminoid-treated groups were significantly lower than the control. Inhibition of rhMMP-9 varied from 29-49% among curcumonoid-treated groups, whereas no inhibition was observed at untreated control (p>0.05). Results were confirmed by zymography. The study showed that the pretreatment of dentin matrices by curcuminoids decreases endogenous protease activity-mediated degradation in dentin.en_US
dc.description.sponsorshipAcademy of Finland [296653]; EVO funding of Turku University Hospital; Finnish Dental Society Research Grant; Academy of Finland (AKA) [296653] Funding Source: Academy of Finland (AKA)en_US
dc.description.sponsorshipThis study was supported by grant #296653 from the Academy of Finland to AT-M (PI), EVO funding of Turku University Hospital to AT-M (PI) and Finnish Dental Society Research Grant to AT-M (PI). The authors do not have a financial interest in products, equipment, and companies cited in the manuscript.en_US
dc.identifier.doi10.4012/dmj.2017-116
dc.identifier.endpage452en_US
dc.identifier.issn0287-4547
dc.identifier.issue3en_US
dc.identifier.pmid29491199en_US
dc.identifier.scopus2-s2.0-85048297697en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage445en_US
dc.identifier.urihttps://doi.org/10.4012/dmj.2017-116
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20361
dc.identifier.volume37en_US
dc.identifier.wosWOS:000439019100012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJapanese Soc Dental Materials Devicesen_US
dc.relation.ispartofDental Materials Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDegradationen_US
dc.subjectCollagen Matrixen_US
dc.subjectCurcuminoidsen_US
dc.subjectDentin Proteasesen_US
dc.subjectDry Mass Lossen_US
dc.subjectMatrix Metalloproteinasesen_US
dc.subjectCathepsin-Hen_US
dc.subjectCollagenen_US
dc.subjectMechanismen_US
dc.subjectMmpsen_US
dc.subjectDesignen_US
dc.subjectCopperen_US
dc.subjectIronen_US
dc.titleInhibitory effect of curcuminoid pretreatments on endogenous dentin proteasesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu