Inhibitory effect of curcuminoid pretreatments on endogenous dentin proteases
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Japanese Soc Dental Materials Devices
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 0/1 of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhIVIMP-9 were measured using an activity assay. Data were subjected to repeated-measures-ANOVA (alpha=0.05). Gelatinolytic activity was analyzed using zymography. ICTP and CTX release and dry mass loss of curcuminoid-treated groups were significantly lower than the control. Inhibition of rhMMP-9 varied from 29-49% among curcumonoid-treated groups, whereas no inhibition was observed at untreated control (p>0.05). Results were confirmed by zymography. The study showed that the pretreatment of dentin matrices by curcuminoids decreases endogenous protease activity-mediated degradation in dentin.
Açıklama
Anahtar Kelimeler
Degradation, Collagen Matrix, Curcuminoids, Dentin Proteases, Dry Mass Loss, Matrix Metalloproteinases, Cathepsin-H, Collagen, Mechanism, Mmps, Design, Copper, Iron
Kaynak
Dental Materials Journal
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
37
Sayı
3
