Evaluation of the selective anticancer potential and the genetic mechanisms of the induction of apoptosis by walnut milk in human breast and prostate cancer cells

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.contributor.authorDoganlar, Oguzhan
dc.contributor.authorDoganlar, Zeynep Banu
dc.date.accessioned2024-06-12T11:07:22Z
dc.date.available2024-06-12T11:07:22Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn different cancer types, classical chemotherapy has several side effects due to the cytotoxic properties of the compounds and non-selective targeting of normal tissue. The aims of this study were to determine bioactive molecules and to investigate the genetic mechanisms of the anticancer properties of walnut special mixture, walnut milk (WM), as a potential anticancer treatment in DU145, MCF7 and TG/HA-VSMC cells. The bioactive molecules of WM were determined by LC-Q-TOF analysis. After treatment with the WM, cell viability was determined using the MTT assay and apoptosis induction was observed following cell membrane staining by annexin-V/propidium-iodide using a Tali-cytometer. The gene expression studies were carried out using a qRT-PCR assay. In the WM, we quantified five hormones, eight polyphenols, quercetin and juglone. Abscisic acid (63.07 +/- 18.70 mu g/l), gallic acid (3887.08 +/- 155.06 mu g/l), quercetin (245.26 +/- 34.12 mu g/l) and juglone (401.52 +/- 16.60 mu g/l) were major components of the quantified compounds. Our results indicated that WM dramatically reduces cell viability and selectively induces caspase-dependent apoptosis in DU145 and MCF7 cells without affecting TG/HA-VSMC non-cancerous cells by triggering intrinsic apoptotic signalling and increases in ROS production. Our results suggest that WM is a potential anticancer agent with selective apoptotic potential and special bioactive chemical constituents.en_US
dc.description.sponsorshipT.R. State Planning Organization [2011K120390]en_US
dc.description.sponsorshipThe authors are grateful to Prof. Dr. Yener YORUK and the Technology Research and Application Centre (TUTAGEM), which is funded by the T.R. State Planning Organization (Project Number: 2011K120390), for providing the laboratory equipment. We acknowledge Ucan Adam (Denizli, Turkey) for supplying us with walnut milk.en_US
dc.identifier.endpage278en_US
dc.identifier.issn0970-938X
dc.identifier.issn0976-1683
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84954515486en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage268en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21995
dc.identifier.volume27en_US
dc.identifier.wosWOS:000374112200047en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAllied Acaden_US
dc.relation.ispartofBiomedical Research-Indiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectJuglans Regia Len_US
dc.subjectWalnut Milken_US
dc.subjectAnticancer Activityen_US
dc.subjectApoptosisen_US
dc.subjectBreast Canceren_US
dc.subjectProstate Canceren_US
dc.subjectJuglans-Regia L.en_US
dc.subjectPhenolic-Compoundsen_US
dc.subjectAntiproliferative Activitiesen_US
dc.subjectAntioxidant Activityen_US
dc.subjectDifferent Cultivarsen_US
dc.subjectOxidative Stressen_US
dc.subjectEllagic Aciden_US
dc.subjectDna-Damageen_US
dc.subjectLineen_US
dc.subjectActivationen_US
dc.titleEvaluation of the selective anticancer potential and the genetic mechanisms of the induction of apoptosis by walnut milk in human breast and prostate cancer cellsen_US
dc.typeArticleen_US

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