Evaluation of the selective anticancer potential and the genetic mechanisms of the induction of apoptosis by walnut milk in human breast and prostate cancer cells
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Allied Acad
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In different cancer types, classical chemotherapy has several side effects due to the cytotoxic properties of the compounds and non-selective targeting of normal tissue. The aims of this study were to determine bioactive molecules and to investigate the genetic mechanisms of the anticancer properties of walnut special mixture, walnut milk (WM), as a potential anticancer treatment in DU145, MCF7 and TG/HA-VSMC cells. The bioactive molecules of WM were determined by LC-Q-TOF analysis. After treatment with the WM, cell viability was determined using the MTT assay and apoptosis induction was observed following cell membrane staining by annexin-V/propidium-iodide using a Tali-cytometer. The gene expression studies were carried out using a qRT-PCR assay. In the WM, we quantified five hormones, eight polyphenols, quercetin and juglone. Abscisic acid (63.07 +/- 18.70 mu g/l), gallic acid (3887.08 +/- 155.06 mu g/l), quercetin (245.26 +/- 34.12 mu g/l) and juglone (401.52 +/- 16.60 mu g/l) were major components of the quantified compounds. Our results indicated that WM dramatically reduces cell viability and selectively induces caspase-dependent apoptosis in DU145 and MCF7 cells without affecting TG/HA-VSMC non-cancerous cells by triggering intrinsic apoptotic signalling and increases in ROS production. Our results suggest that WM is a potential anticancer agent with selective apoptotic potential and special bioactive chemical constituents.
Açıklama
Anahtar Kelimeler
Juglans Regia L, Walnut Milk, Anticancer Activity, Apoptosis, Breast Cancer, Prostate Cancer, Juglans-Regia L., Phenolic-Compounds, Antiproliferative Activities, Antioxidant Activity, Different Cultivars, Oxidative Stress, Ellagic Acid, Dna-Damage, Line, Activation
Kaynak
Biomedical Research-India
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
27
Sayı
1
