The effects of sildenafil and n-acetylcysteine on ischemia and reperfusion injury in gastrocnemius muscle and femoral artery endothelium

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dc.authoridSunar, Hasan/0000-0002-1276-8549
dc.authoridiscan, sahin/0000-0003-1015-5260
dc.authoridYuksel, Volkan/0000-0001-9518-2588
dc.authoridHuseyin, Serhat/0000-0003-4118-040X
dc.authoridsagiroglu, gonul/0000-0002-1189-4973
dc.authorwosidSunar, Hasan/A-8685-2018
dc.authorwosidSagiroglu, Gonul/ABH-1345-2021
dc.authorwosidsagiroglu, gönül/AAB-4472-2021
dc.contributor.authorAksu, Volkan
dc.contributor.authorYuksel, Volkan
dc.contributor.authorChousein, Serchat
dc.contributor.authorTastekin, Ebru
dc.contributor.authorIscan, Sahin
dc.contributor.authorSagiroglu, Gonul
dc.contributor.authorCanbaz, Suat
dc.date.accessioned2024-06-12T11:01:51Z
dc.date.available2024-06-12T11:01:51Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose We aimed to examine the effects of sildenafil and n-acetylcystein on ischemia/reperfusion injury in femoral artery endothelium and gastrocnemius muscle. Basic methods 32 rats of Sprague-Dawley breed were randomly divided into four groups (n=8). Median laparotomy was performed, then a 120-minute ischemia was created by microvascular clamping of infrarenal aorta, followed by the release of clamping. In sildenafil group, 1mg/kg of sildenafil infusion and in the n-acetylcystein group, 100mg/kg of n-acetylcystein infusion was administered after release of clamps. Blood samples and tissue samples of femoral artery and gastrocnemius muscle were extracted for a histopathological evaluation. Principal findings Serum levels of malondialdehyde in ischemia/reperfusion group (6.160.79) were higher compared to the control group (4.69 +/- 0.33), whereas a significant decrease was detected in sildenafil (5.17 +/- 0.50) and n-acetylcystein (4.96 +/- 0.49) groups. Femoral artery tissue sections of the control group, mean tumor necrosis factor alpha and hypoxy-induced factor-1 alpha immunoreactivity were found to be negative. In the ischemia/reperfusion group, mean tumor necrosis factor immunoreactivity was intense and mean hypoxy-induced factor-1 alpha immunoreactivity was 51-75%. In the ischemia/reperfusion+Sildenafil and ischemia/reperfusion+NAS groups, mean tumor necrosis factor immunoreactivity was slight and mean hypoxy-induced factor-1 alpha immunoreactivity was 26-50%. Conclusions In conclusion, sildenafil and n-acetylcystein may reduce femoral artery endothelium and gastrocnemius muscle injury following lower extremity ischemia/reperfusion.en_US
dc.description.sponsorshipTrakya University Scientific Research Departmenten_US
dc.description.sponsorshipThe study was funded by Trakya University Scientific Research Department.en_US
dc.identifier.doi10.1177/1708538114527939
dc.identifier.endpage30en_US
dc.identifier.issn1708-5381
dc.identifier.issn1708-539X
dc.identifier.issue1en_US
dc.identifier.pmid24642934en_US
dc.identifier.scopus2-s2.0-84920972762en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.1177/1708538114527939
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21055
dc.identifier.volume23en_US
dc.identifier.wosWOS:000348102300004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofVascularen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFemoral Artery Endotheliumen_US
dc.subjectGastrocnemius Muscleen_US
dc.subjectIschemiaen_US
dc.subjectN-Acetylcysteinen_US
dc.subjectReperfusionen_US
dc.subjectSildenafilen_US
dc.subjectDysfunctionen_US
dc.subjectPathophysiologyen_US
dc.subjectModelen_US
dc.titleThe effects of sildenafil and n-acetylcysteine on ischemia and reperfusion injury in gastrocnemius muscle and femoral artery endotheliumen_US
dc.typeArticleen_US

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