The effects of sildenafil and n-acetylcysteine on ischemia and reperfusion injury in gastrocnemius muscle and femoral artery endothelium
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Sage Publications Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose We aimed to examine the effects of sildenafil and n-acetylcystein on ischemia/reperfusion injury in femoral artery endothelium and gastrocnemius muscle. Basic methods 32 rats of Sprague-Dawley breed were randomly divided into four groups (n=8). Median laparotomy was performed, then a 120-minute ischemia was created by microvascular clamping of infrarenal aorta, followed by the release of clamping. In sildenafil group, 1mg/kg of sildenafil infusion and in the n-acetylcystein group, 100mg/kg of n-acetylcystein infusion was administered after release of clamps. Blood samples and tissue samples of femoral artery and gastrocnemius muscle were extracted for a histopathological evaluation. Principal findings Serum levels of malondialdehyde in ischemia/reperfusion group (6.160.79) were higher compared to the control group (4.69 +/- 0.33), whereas a significant decrease was detected in sildenafil (5.17 +/- 0.50) and n-acetylcystein (4.96 +/- 0.49) groups. Femoral artery tissue sections of the control group, mean tumor necrosis factor alpha and hypoxy-induced factor-1 alpha immunoreactivity were found to be negative. In the ischemia/reperfusion group, mean tumor necrosis factor immunoreactivity was intense and mean hypoxy-induced factor-1 alpha immunoreactivity was 51-75%. In the ischemia/reperfusion+Sildenafil and ischemia/reperfusion+NAS groups, mean tumor necrosis factor immunoreactivity was slight and mean hypoxy-induced factor-1 alpha immunoreactivity was 26-50%. Conclusions In conclusion, sildenafil and n-acetylcystein may reduce femoral artery endothelium and gastrocnemius muscle injury following lower extremity ischemia/reperfusion.
Açıklama
Anahtar Kelimeler
Femoral Artery Endothelium, Gastrocnemius Muscle, Ischemia, N-Acetylcystein, Reperfusion, Sildenafil, Dysfunction, Pathophysiology, Model
Kaynak
Vascular
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
23
Sayı
1
