Osteoblast behaviour on zirconia fabricated by additive and subtractive technology
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Zirconia is an attractive material for the manufacturing of oral implants and patient-individual barriers for guided bone regeneration. This study compared osteoblast behaviour on additive manufactured and milled 3-mol % yttria-stabilized tetragonal polycrystal zirconia (ZrO2).Two groups of samples (O5 x 2 mm) were manufactured by stereolithography (LithaCon 230, Lithoz, Vienna, Austria) and subsequently heat-treated and sintered. One group remained superficially unmodified (AM-ZrO2unmod.), while the other was polished according to DIN EN ISO 6872 standard using automated grinding (AMZrO2-pol.). For comparison, milled samples (e.max ZirCAD LT, Ivoclar Vivadent, Schaan, Liechtenstein) were sintered and polished similarly (Mil-ZrO2-pol.). Surface roughness was characterized using tactile profilometry. Adhesion, proliferation and coverage of human fetal osteoblasts (hFOB 1.19) as well as expression of osteogenic marker genes ALPL and RUNX2 were determined (and statistically compared using Kruskal-Wallis-analyses and Dunn's Post-hoc-tests).The surface roughness of AM-ZrO2-unmod. Was 45 times higher than that of the polished groups. No significant differences were detected between the three groups regarding cell adhesion and proliferation. With regard to cell coverage, AM-ZrO2-pol. Significantly outperformed the two other groups (p < 0.05). ALPL- and RUNX2mRNA expression was insignificantly superior for AM-ZrO2-unmod. Compared to the two other groups. Therefor no detrimental effect on osteoblast behaviour caused by the investigated, acrylic binder-based 3D printing workflow was observed.
Açıklama
Anahtar Kelimeler
Additive Manufacturing, Zirconia, Implant, 3D Printing, CAD, CAM, Surface-Topography, Molar Replacement, Dental Implants, In-Vitro, Titanium, Immediate, Cad/Cam, Ceramics
Kaynak
Ceramics International
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
49
Sayı
6
