Role of Ovarian Hormones in Psychological Stress-induced Oxidative Organ Damage in Rats
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aves Press Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: Stress response varies with respect to gender via the hypothalamic-pituitary-gonadal axis. We aimed to investigate the effect of ovarian hormone deficiency on psychological stress response and oxidative damage. Methods: Female Sprague Dawley rats (250-300 g, n=56) were divided as control, sham, and ovariectomy (OVX) groups. Sham operation or surgical OVX were conducted under anesthesia. After 60 days, the rats were placed in a special chamber to induce psychological stress by electric shock and were kept in the same chamber for 30 min on the following 3 days. Glucocorticoid receptor antagonist RU-486 (10 mg/kg), oxytocin receptor antagonist atosiban (1 mg/kg), or saline was intraperitoneally administered 10 min before stress exposure. After the hole-board anxiety test, the rats were decapitated on the 4th day; tissue and blood samples were obtained. Results: Psychological stress increased cortisol levels in the RU-486-administered group, while cortisol levels were decreased in the atosiban-administered group. Serum interleukin (IL)-1 ss levels, but not TNF-alpha levels, were increased by inducing stress. Stress increased oxidative damage in the stomach, colon, and brain of ovariectomized rats (p<0.05-0.001), while atosiban partially reversed and RU-486 exaggerated oxidative damage. GSH levels that were depleted because of stress were partially replenished by administering atosiban; however, RU-486 had no effect on GSH levels. Conclusion: Although the absence of ovarian hormones during psychological stress had no effect on cortisol or anxiety levels, changes in cytokine levels and oxidative tissue damage were observed.
Açıklama
Anahtar Kelimeler
Hypothalamopituitary-Adrenal Axis, Hypothalamopituitary Gonadal Axis, Glucocorticoid Receptor, Atosiban, Oxytocin
Kaynak
Clinical And Experimental Health Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
6
Sayı
2
