Comparison off some properties of free and immobilized ?-amylase by Aspergillus sclerotiorum in calcium alginate gel beads

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dc.authoridErtan, Figen/0000-0002-7882-3993
dc.contributor.authorYagar, Hulya
dc.contributor.authorErtan, Figen
dc.contributor.authorBalkan, Bilal
dc.date.accessioned2024-06-12T10:51:19Z
dc.date.available2024-06-12T10:51:19Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractSome properties of immobilized a-amylase by Aspergillus sclerotiorum within calcium alginate gel beads were investigated and compared with soluble enzyme. Optimum pH and temperature were found to be 5.0 and 40 degrees C, respectively, for both soluble and immobilized enzymes. The immobilized enzyme had a better Km value, but k(cat)/K-m values were the same for both enzymes. Entrapment within calcium alginate gel beads improved, remarkably, the thermal and storage stability of a-amylase. The half life values of immobilized enzyme and soluble enzyme at 60 degrees C were 164.2, and 26.2 min, respectively. The midpoint of thermal inactivation (T-m) shifted from 56 degrees C (for soluble enzyme) to 65.4 degrees C for immobilized enzyme. The percentages of soluble starch hydrolysis for soluble and immobilized alpha-amylase were determined to be 97.5 and 92.2% for 60 min, respectively.en_US
dc.identifier.doi10.1080/10826060701774304
dc.identifier.endpage23en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue1en_US
dc.identifier.pmid18080907en_US
dc.identifier.scopus2-s2.0-37249017029en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage13en_US
dc.identifier.urihttps://doi.org/10.1080/10826060701774304
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18311
dc.identifier.volume38en_US
dc.identifier.wosWOS:000252312100002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectAspergillus Sclerotiorumen_US
dc.subjectEntrapmenten_US
dc.subjectCalcium Alginateen_US
dc.subjectSoluble Starchen_US
dc.subjectHydrolysisen_US
dc.subjectOptimizationen_US
dc.subjectHydrolysisen_US
dc.subjectStarchen_US
dc.titleComparison off some properties of free and immobilized ?-amylase by Aspergillus sclerotiorum in calcium alginate gel beadsen_US
dc.typeArticleen_US

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