The Effects of Micronuclei with Whole Chromosome on Biological Dose Estimation

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dc.contributor.authorPala, Funda S.
dc.contributor.authorAlkaya, Fadime
dc.contributor.authorTabakcioglu, Kiymet
dc.contributor.authorTokatli, Fuesun
dc.contributor.authorUzal, Cem
dc.contributor.authorParlar, Sule
dc.contributor.authorAlguenes, Cetin
dc.date.accessioned2024-06-12T10:54:37Z
dc.date.available2024-06-12T10:54:37Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe total micronucleus (MN) assay has been used for purposes of biological dosimetry for many years. The variable spontaneous incidence of micronuclei in peripheral blood lymphocytes affects the sensitivity of biological dose estimations at low doses. It has been suggested that this problem could be solved by using the micronuclei-centromere assay. In this study. Co-60 gamma ray dose response curves for micronuclei (MN) and micronuclei without centromeres (MNC-) in the range of 0-5.0 Gy were established using a pancentromeric FISH probe on cultured binucleate lymphocytes from 2 donors. There were no significant inter-donor differences in the dose responses for either MN or MNC-. The relative proportions of MN that contained centromeres (MNC+) decreased with radiation dose, which is in line with the proposition that radiation predominately causes chromosomal breakage rather than whole chromosome loss. The a coefficients of MNC- curves decreased to 62% of the values for total MN whilst the beta coefficients were unchanged. MN and MNC+ frequencies of 60 control smoker and 40 non-smoker donors were also compared. No effect of smoking was observed. However the MNC+ spontaneous frequencies showed an age and gender effect with the highest frequencies in older women.en_US
dc.identifier.endpage290en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-56049084891en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage283en_US
dc.identifier.trdizinid81366en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/81366
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19109
dc.identifier.volume32en_US
dc.identifier.wosWOS:000261624000009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiodosimetryen_US
dc.subjectMicronucleien_US
dc.subjectMicronuclei-Centromere Assayen_US
dc.subjectCo-60 Gamma Dose Response Curveen_US
dc.subjectCytokinesis-Blocked Lymphocytesen_US
dc.subjectCentromere Assayen_US
dc.subjectIonizing-Radiationen_US
dc.subjectFrequencyen_US
dc.subjectHybridizationen_US
dc.subjectSmokingen_US
dc.subjectSmokersen_US
dc.subjectProjecten_US
dc.subjectDamageen_US
dc.titleThe Effects of Micronuclei with Whole Chromosome on Biological Dose Estimationen_US
dc.typeArticleen_US

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