The Effect of Phosphate Limitation on Rhamnolipid Production and Related Quorum Sensing Signal Molecules in Pseudomonas aeruginosa

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dc.authoridBukavaz, Sebnem/0000-0001-7460-8922
dc.authorwosidBukavaz, Sebnem/IYJ-6961-2023
dc.authorwosidBukavaz, Sebnem/AAA-2957-2020
dc.authorwosidBukavaz, Sebnem/AAH-9346-2019
dc.contributor.authorBukavaz, Sebnem
dc.date.accessioned2024-06-12T11:13:04Z
dc.date.available2024-06-12T11:13:04Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIntroduction: Rhamnolipid (RL) is one of the virulence factor secreted from Pseudomonas aeruginosa and is required to establish and persist the infection. The control of RL production is under control of Quorum Sensing System, and it is influenced by numerous factors of environmental/nutritional levels. In this study, it was aimed to investigated the phosphate depletion effect on selected Quorum Sensing Signal Molecules (QSSMs) under condition of rhamnolipid production. Materials and Methods: The effect of phosphate on RL production was examined in P. aeruginosa PAO1 and RL mutant strains by Orcinol and TLC plate test. Additionally, HPLC/MS quantification for QSSMs related to rhamnolipid production was carried out using the samples isolated from 16, 24 and 48 h cultures grown in Luria-Bertani (LB) and Proteose Peptone Glucose Amonium Salt (PPGAS) medium. Results: The increase in rhamnolipid production in PPGAS medium, for the wild type strain (48 h) amounted up to 20 fold was achieved compared to LB media by Orcinol assay. The cultures from PPGAS medium supplemented with 50 mM phosphate displayed significantly lower amount of rhamnolipid in 48 and 72 h cultures by 54% and 64%, respectively. The highest concentration of QSSMs were the C4-HSL and PQS for all strains. Then, the QSSM profile was followed by 3-oxo-C12-HSL and HHQ. Conclusion: Phosphate limitation increased rhamnolipid production via RhlR and PQS systems in P. aeruginosa PAO1. Considering the fact that other virulence factors are activated while rhamnolipid production is increased, phosphate limitation leads to an increase in the virulence of P. aeruginosa.en_US
dc.identifier.doi10.5578/flora.67349
dc.identifier.endpage100en_US
dc.identifier.issn1300-932X
dc.identifier.issue2en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://doi.org/10.5578/flora.67349
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23402
dc.identifier.volume24en_US
dc.identifier.wosWOS:000473317400003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherBilimsel Tip Yayinevien_US
dc.relation.ispartofFlora Infeksiyon Hastaliklari Ve Klinik Mikrobiyoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPseudomonas Aeruginosaen_US
dc.subjectRhamnolipiden_US
dc.subjectQuorum Sensing Signal Moleculesen_US
dc.subjectPhosphate Limitationen_US
dc.subjectC4-HSLen_US
dc.subject3-Oxo-C12-HSLen_US
dc.subjectHHQen_US
dc.subjectPQSen_US
dc.subjectQuinolone Signalen_US
dc.subjectHierarchyen_US
dc.subjectRhlren_US
dc.subjectVirulenceen_US
dc.subjectGenomeen_US
dc.subjectGenesen_US
dc.titleThe Effect of Phosphate Limitation on Rhamnolipid Production and Related Quorum Sensing Signal Molecules in Pseudomonas aeruginosaen_US
dc.typeArticleen_US

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