The Effect of Phosphate Limitation on Rhamnolipid Production and Related Quorum Sensing Signal Molecules in Pseudomonas aeruginosa
Küçük Resim Yok
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bilimsel Tip Yayinevi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: Rhamnolipid (RL) is one of the virulence factor secreted from Pseudomonas aeruginosa and is required to establish and persist the infection. The control of RL production is under control of Quorum Sensing System, and it is influenced by numerous factors of environmental/nutritional levels. In this study, it was aimed to investigated the phosphate depletion effect on selected Quorum Sensing Signal Molecules (QSSMs) under condition of rhamnolipid production. Materials and Methods: The effect of phosphate on RL production was examined in P. aeruginosa PAO1 and RL mutant strains by Orcinol and TLC plate test. Additionally, HPLC/MS quantification for QSSMs related to rhamnolipid production was carried out using the samples isolated from 16, 24 and 48 h cultures grown in Luria-Bertani (LB) and Proteose Peptone Glucose Amonium Salt (PPGAS) medium. Results: The increase in rhamnolipid production in PPGAS medium, for the wild type strain (48 h) amounted up to 20 fold was achieved compared to LB media by Orcinol assay. The cultures from PPGAS medium supplemented with 50 mM phosphate displayed significantly lower amount of rhamnolipid in 48 and 72 h cultures by 54% and 64%, respectively. The highest concentration of QSSMs were the C4-HSL and PQS for all strains. Then, the QSSM profile was followed by 3-oxo-C12-HSL and HHQ. Conclusion: Phosphate limitation increased rhamnolipid production via RhlR and PQS systems in P. aeruginosa PAO1. Considering the fact that other virulence factors are activated while rhamnolipid production is increased, phosphate limitation leads to an increase in the virulence of P. aeruginosa.
Açıklama
Anahtar Kelimeler
Pseudomonas Aeruginosa, Rhamnolipid, Quorum Sensing Signal Molecules, Phosphate Limitation, C4-HSL, 3-Oxo-C12-HSL, HHQ, PQS, Quinolone Signal, Hierarchy, Rhlr, Virulence, Genome, Genes
Kaynak
Flora Infeksiyon Hastaliklari Ve Klinik Mikrobiyoloji Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
24
Sayı
2
