Effects of Bevacizumab, Ranibizumab, and Aflibercept on MicroRNA Expression in a Retinal Pigment Epithelium Cell Culture Model of Oxidative Stress

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dc.authorwosidAyaz, Lokman/K-6716-2013
dc.contributor.authorDinc, Erdem
dc.contributor.authorAyaz, Lokman
dc.contributor.authorKurt, Akif Hakan
dc.date.accessioned2024-06-12T11:08:30Z
dc.date.available2024-06-12T11:08:30Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: This study aimed to evaluate the effects of bevacizumab, ranibizumab, and aflibercept on the microRNA (miRNA) expression in human retinal pigment epithelium cell (ARPE-19) culture model of oxidative stress. Methods: Control cells were cultured in the hydrogen peroxide (H2O2)-free medium. In H2O2 group ARPE-19 cells were exposed to 600M H2O2 alone for 18h. In study groups, cells were preincubated with bevacizumab, ranibizumab, and aflibercept (1.25-2.5, 0.5 and 2.0mg/mL, respectively) for 3h before H2O2 exposure. Another group of ARPE-19 cells were incubated with drugs for 3h without H2O2 exposure. Cell viability and vascular endothelial growth factor (VEGF) levels were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and enzyme-linked immunosorbent assay. The expression levels of 1,152 miRNAs were determined by quantitative real-time PCR. Results: Incubation with 600M H2O2 alone for 18h decreased cell viability by approximate to 50%. Cell viability was greater in the anti-VEGF drug groups compared with the H2O2 group, but the differences were not significant (P>0.05). VEGF levels were significantly lower in the anti-VEGF drug groups compared with the H2O2 group (P<0.05 for all study groups), with no significant differences between the study groups (P>0.05). Incubation with anti-VEGF drugs alone had no effect on miRNA expression in ARPE-19 cells. However, preincubation with bevacizumab, ranibizumab, and aflibercept significantly altered the profile of H2O2-modulated miRNA expression. Conclusions: Preincubation with anti-VEGF drugs can alter the miRNA expression profile in response to H2O2-induced oxidative stress, and these drugs may have epigenetic effects.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S790]en_US
dc.description.sponsorshipThe authors thank to Yilmaz N for supporting cell culture studies. This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Grant No. 114S790).en_US
dc.identifier.doi10.1089/jop.2017.0128
dc.identifier.endpage353en_US
dc.identifier.issn1080-7683
dc.identifier.issn1557-7732
dc.identifier.issue4en_US
dc.identifier.pmid29389239en_US
dc.identifier.scopus2-s2.0-85046907666en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage346en_US
dc.identifier.urihttps://doi.org/10.1089/jop.2017.0128
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22464
dc.identifier.volume34en_US
dc.identifier.wosWOS:000424083000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofJournal Of Ocular Pharmacology And Therapeuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrornaen_US
dc.subjectBevacizumaben_US
dc.subjectRanibizumaben_US
dc.subjectAflibercepten_US
dc.subjectARPE-19 Cellsen_US
dc.subjectOxidative Stressen_US
dc.subjectMirnasen_US
dc.subjectGenesen_US
dc.titleEffects of Bevacizumab, Ranibizumab, and Aflibercept on MicroRNA Expression in a Retinal Pigment Epithelium Cell Culture Model of Oxidative Stressen_US
dc.typeArticleen_US

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