Effects of Bevacizumab, Ranibizumab, and Aflibercept on MicroRNA Expression in a Retinal Pigment Epithelium Cell Culture Model of Oxidative Stress
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mary Ann Liebert, Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: This study aimed to evaluate the effects of bevacizumab, ranibizumab, and aflibercept on the microRNA (miRNA) expression in human retinal pigment epithelium cell (ARPE-19) culture model of oxidative stress. Methods: Control cells were cultured in the hydrogen peroxide (H2O2)-free medium. In H2O2 group ARPE-19 cells were exposed to 600M H2O2 alone for 18h. In study groups, cells were preincubated with bevacizumab, ranibizumab, and aflibercept (1.25-2.5, 0.5 and 2.0mg/mL, respectively) for 3h before H2O2 exposure. Another group of ARPE-19 cells were incubated with drugs for 3h without H2O2 exposure. Cell viability and vascular endothelial growth factor (VEGF) levels were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and enzyme-linked immunosorbent assay. The expression levels of 1,152 miRNAs were determined by quantitative real-time PCR. Results: Incubation with 600M H2O2 alone for 18h decreased cell viability by approximate to 50%. Cell viability was greater in the anti-VEGF drug groups compared with the H2O2 group, but the differences were not significant (P>0.05). VEGF levels were significantly lower in the anti-VEGF drug groups compared with the H2O2 group (P<0.05 for all study groups), with no significant differences between the study groups (P>0.05). Incubation with anti-VEGF drugs alone had no effect on miRNA expression in ARPE-19 cells. However, preincubation with bevacizumab, ranibizumab, and aflibercept significantly altered the profile of H2O2-modulated miRNA expression. Conclusions: Preincubation with anti-VEGF drugs can alter the miRNA expression profile in response to H2O2-induced oxidative stress, and these drugs may have epigenetic effects.
Açıklama
Anahtar Kelimeler
Microrna, Bevacizumab, Ranibizumab, Aflibercept, ARPE-19 Cells, Oxidative Stress, Mirnas, Genes
Kaynak
Journal Of Ocular Pharmacology And Therapeutics
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
34
Sayı
4
