DNA methylation of the prestin gene and outer hair cell electromotile response of the cochlea in salicylate administration
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background/aim: Activity of the prestin gene may have a role in the pathogenesis of salicylate-induced ototoxicity. We investigated DNA methylation for prestin gene exon 1 in salicylate-injected guinea pigs. Materials and methods: Fifteen guinea pigs (30 ears) underwent audiological evaluation including 1000 Hz probe-tone tympanometry and a distortion product otoacoustic emission (DPOAE) test. The animals were randomly divided into three groups. Groups 2 (8 ears) and 3 (14 ears) were injected with intramuscular saline and sodium salicylate (200 mg/kg), respectively twice daily for 2 weeks. Group 1 (8 ears) received no injection. DPOAE measurements were performed at baseline; after 1, 2, 4, and 8 h (acute effect); and after 1 and 2 weeks (chronic effect). After audiological measurements, the animals were sacrificed for DNA isolation. Results: While a significant decrease (P < 0.01) was found for the acute effect in all frequencies in Group 3 according to baseline measurements, there was no difference in terms of chronic effect. DNA methylation increased during the acute phase of salicylate administration, whereas it returned to initial levels during the chronic phase. Conclusion: Salicylate-induced changes in DPOAE responses may be related to prestin-gene methylation. These results may have important implications for salicylate ototoxicity.
Açıklama
36th Turkish National Meeting of the Otorhinolaryngology and Head and Neck Surgery -- NOV 05-09, 2014 -- Antalya, TURKEY
Anahtar Kelimeler
Ototoxicity, Tinnitus, Hair Cells, Auditory, Outer, Distortion Product Otoacoustic Emission, Sodium Salicylate, DNA Methylation, Non-Cpg Methylation, Auditory-Perception Tinnitus, Embryonic Stem-Cells, Motor Protein, Guinea-Pig, Mechanisms, Expression, Aspirin, Rats
Kaynak
Turkish Journal Of Medical Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
47
Sayı
5
