Protective effect of sildenafil on liver injury induced by intestinal ischemia/reperfusion
Küçük Resim Yok
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
W B Saunders Co-Elsevier Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background: This study evaluated the protective effect of sildenafil on liver injury induced by intestinal ischemia-reperfusion. Methods: Forty female Sprague Dawley rats were divided into 4 groups: sham-control (SC), ischemia (I), ischemia-reperfusion (IR), and ischemia-reperfusion+ sildenafil (SIL; sildenafil gavaged at 50 mg/kg before operating). A 2-h ischemia-reperfusion was performed by clamping the superior mesenteric artery. Liver function, plasma alanine (ALT) and aspartate (AST) aminotransferase, and intestinal and liver malondialdehyde (MDA) were measured at the end of the experiment. Intestinal and liver tissue damage was examined by histology. Liver samples were immunologically stained for endothelial nitric oxide synthase (eNOS) and proliferating cell nuclear antigen (PCNA). Results: The ALT and AST levels were highest in the IR group and were lower in the SIL group (p<0.05). Intestinal MDA levels were statistically higher in the IR group than in the SC, I and SIL groups. Liver MDA levels were significantly higher in the IR group than in the I and SC groups (p<0.05) and higher than in the SIL group (p>0.05). Intestinal damage based on Chiu scoring was more severe in the IR than in the SIL group (p<0.05). Sildenafil reduced damage and also increased eNOS and PCNA immunoreactivity in liver tissue. Conclusions: Sildenafil shows a protective effect on intestinal ischemia-reperfusion-induced liver injury, possibly by decreasing vascular resistance through increased nitric oxide levels. (C) 2013 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Sildenafil, Intestine, Ischemia-Reperfusion, Liver, Intestinal Ischemia/Reperfusion-Induced Liver Injury, Ischemia-Reperfusion Injury, Nitric-Oxide, Lung Injury, Rat-Liver, Dysfunction, Mechanisms, Vardenafil, Cirrhosis, Citrate, Mice
Kaynak
Journal Of Pediatric Surgery
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
48
Sayı
8
