Molecular Identification of Campylobacter Species Isolated from Patients with Gastroenteritis in Edirne, Turkey

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dc.authorwosidBukavaz, Sebnem/IYJ-6961-2023
dc.contributor.authorEryildiz, Canan
dc.contributor.authorSakru, Nermin
dc.contributor.authorTabakcioglu, Kiymet
dc.contributor.authorUgur, Mediha Cerrah
dc.contributor.authorBukavaz, Sebnem
dc.date.accessioned2024-06-12T10:55:31Z
dc.date.available2024-06-12T10:55:31Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBACKGROUND/AIMS: Campylobacter is a major cause of foodborne diarrheal disease, and the incidence of campylobacteriosis has significantly increased in both developed and developing countries. The purpose of the present study was to identify the species of Campylobacter isolates and to evaluate the distribution of Campylobacter infections according to various characteristics in our region. MATERIALS AND METHODS: Campylobacter isolates obtained from patients at a tertiary hospital in Edirne, Turkey were included in this study. The distribution of Campylobacter infections was evaluated according to age, season, and gender. Species identification was performed by multiplex polymerase chain reaction (PCR). The RNA polymerase beta-subunit gene (rpoB) of selected samples was amplified, and DNA sequencing was performed. RESULTS: Campylobacter species were isolated from 226 (4.3%) of the 5,241 samples. One hundred and seventy-six (89.3%) of 197 samples were identified as C. jejuni and 19 (9.6%) as C. coli by multiplex PCR. Two isolates showed a band profile compatible with both C. jejuni and C. coli. DNA sequencing was performed for 21 isolates. Sixteen isolates were compatible with C. jejuni and 5 isolates were consistent with C. coli. There was no statistically significant difference in Campylobacter isolation rates according to gender and season (p>0.05). Campylobacter species were most frequently isolated from children in the age group of 0-14 years (p<0.01). CONCLUSION: Campylobacter is one of the main causes of diarrhea in Turkey, and this infection is more common in children. This study contributes to information about the situation of Campylobacter infection and the genetic features of isolates in Turkey.en_US
dc.description.sponsorshipTrakya University Scientific Research Fund; [TUBAP-2017/53]en_US
dc.description.sponsorshipFinancial Disclosure: This study was financially supported by the Trakya University Scientific Research Fund [TUBAP-2017/53] .en_US
dc.identifier.doi10.4274/cjms.2022.2021-164
dc.identifier.endpage627en_US
dc.identifier.issn2149-7893
dc.identifier.issn2536-507X
dc.identifier.issue5en_US
dc.identifier.startpage623en_US
dc.identifier.trdizinid1166263en_US
dc.identifier.urihttps://doi.org/10.4274/cjms.2022.2021-164
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1166263
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19459
dc.identifier.volume7en_US
dc.identifier.wosWOS:000886060300009en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofCyprus Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCampylobacteren_US
dc.subjectMultiplex PCRen_US
dc.subjectDNA Sequencingen_US
dc.subjectTurkeyen_US
dc.subjectEpidemiologyen_US
dc.subjectInfectionsen_US
dc.subjectResistanceen_US
dc.subjectPrevalenceen_US
dc.subjectJejunien_US
dc.subjectColien_US
dc.titleMolecular Identification of Campylobacter Species Isolated from Patients with Gastroenteritis in Edirne, Turkeyen_US
dc.typeArticleen_US

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