Phytochemical Characterization of Phenolic Compounds by LC-MS/MS and Biological Activities of Ajuga reptans L., Ajuga salicifolia (L.) Schreber and Ajuga genevensis L. from Turkey

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dc.authoridKöse, Yavuz Bülent/0000-0002-3060-7271
dc.authoridGoger, Gamze/0000-0003-2978-5385
dc.authoridKöse, Yavuz Bülent/0000-0002-3060-7271
dc.authoridGöger, Fatih/0000-0002-9665-0256
dc.authoridDemirci, Fatih/0000-0003-1497-3017
dc.authorwosidKöse, Yavuz Bülent/IZP-8734-2023
dc.authorwosidGoger, Gamze/JCE-6911-2023
dc.authorwosidKöse, Yavuz Bülent/H-1215-2015
dc.authorwosidGöger, Fatih/HTR-3454-2023
dc.authorwosidDemirci, Fatih/A-8295-2008
dc.contributor.authorGoger, Gamze
dc.contributor.authorKose, Yavuz Bulent
dc.contributor.authorDemirci, Fatih
dc.contributor.authorGoger, Fatih
dc.date.accessioned2024-06-12T10:51:05Z
dc.date.available2024-06-12T10:51:05Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjectives: In this study, it was aimed to characterize the phenolic contents of Ajuga reptans L., Ajuga salicifolia (L.) Schreber and Ajuga genevensis L. and to investigate their in vitro antioxidant and antimicrobial activities. Materials and Methods: Air dried aerial parts of A. reptans L., A. salicifolia (L.) Schreber, and A. genevensis L. collected from Turkey were extracted with methanol (70%), and the phenolic composition of the crude extracts was analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) method. To determine the total phenolic content the Folin-Ciocalteu method was used. The radical scavenging activities of the extracts were evaluated by the photometric 1,1-diphenyl-2-picrylhydrazyl radical, and trolox equivalent antioxidant capacity assays (TEAC). Furthermore, Ajuga sp. extracts were tested against Escherichia coli NRRL B3008, Staphylococcus areus ATCC 6538, Salmonella thyphimurium ATCC 13311, Bacillus cereus NRRL B-3711, Candida albicans ATCC 90028, Candida tropicalis ATCC 1369, and Candida parapsilosis ATCC 22019 using the in vitro broth dilution assay. Results: The LC-MS/MS analyses identified 19 compounds. The amount of total phenolics ranged from 30.0 to 42.2 mg gallic acid equivalent/g in all extracts. According to the results of TEAC assay, the tested extracts were found to have relatively high activity at 1.2-1.5 mM concentrations. Ajuga sp. extracts inhibited all tested microorganisms; however, C. albicans, C. tropicalis, and C. parapsilosis exhibited relatively more susceptibility (minimum inhibitory concentration: 156.25 mu g/mL) compared to the bacteria tested. Conclusion: The antioxidant activities of all extracts were determined for the first time by the TEAK method, and the in vitro antimicrobial activity of A. salicifolia was investigated for the first time against selected strains.en_US
dc.description.sponsorshipAnadolu University [BAP: 080307]en_US
dc.description.sponsorshipThis work was supported by the Anadolu University Research Funding (project number: BAP: 080307).en_US
dc.identifier.doi10.4274/tjps.galenos.2021.33958
dc.identifier.endpage627en_US
dc.identifier.issn1304-530X
dc.identifier.issn2148-6247
dc.identifier.issue5en_US
dc.identifier.pmid34719190en_US
dc.identifier.scopus2-s2.0-85118503344en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage616en_US
dc.identifier.trdizinid483107en_US
dc.identifier.urihttps://doi.org/10.4274/tjps.galenos.2021.33958
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/483107
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18240
dc.identifier.volume18en_US
dc.identifier.wosWOS:000756366800013en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofTurkish Journal Of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAjuga Reptansen_US
dc.subjectAjuga Salicifoliaen_US
dc.subjectAjuga Genevensisen_US
dc.subjectLC-MS/MSen_US
dc.subjectAntioxidant Activityen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectAntiinflammatory Activitiesen_US
dc.subjectIridoid Glucosidesen_US
dc.subjectIn-Vitroen_US
dc.subjectPlantsen_US
dc.subjectAntibacterialen_US
dc.subjectAntioxidanten_US
dc.subjectGlycosidesen_US
dc.subjectPhytoecdysteroidsen_US
dc.subjectAnthocyaninsen_US
dc.subjectPolyphenolsen_US
dc.titlePhytochemical Characterization of Phenolic Compounds by LC-MS/MS and Biological Activities of Ajuga reptans L., Ajuga salicifolia (L.) Schreber and Ajuga genevensis L. from Turkeyen_US
dc.typeArticleen_US

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