Multifaceted impact of adipose conditioned media: Obesity-driven promotion of prostate cancer and cancer stem cell dynamics

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dc.authoridSERTTAS, RIZA/0000-0002-7493-0388
dc.authoridDIBIRDIK, ILKER/0000-0002-6892-8222
dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.authorwosidSERTTAS, RIZA/AAG-7463-2020
dc.contributor.authorErdogan, Suat
dc.contributor.authorSerttas, Riza
dc.contributor.authorDibirdik, Ilker
dc.contributor.authorTurkekul, Kader
dc.date.accessioned2024-06-12T10:50:45Z
dc.date.available2024-06-12T10:50:45Z
dc.date.issued2024
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObesity is an established risk factor for the development and progression of prostate cancer (PC). This study used adipose conditioned media (ACM) from differentiated adipocytes to assess its effect on PC development and aggressiveness. Due to limited research on ACM's impact on isolated PC stem cells (PCSCs), we also examined CD44+ PCSCs. ACM notably boosted interleukin-1 beta (IL-1 beta), IL-6, and IL-8 production in normal prostate epithelial cells and LNCaP cells. It also increased IL-6 and IL-8 production in PC3 and CD44+ LNCaP cells, and IL-1 beta and IL-6 production in CD44+ PC3 cells. This indicates that ACM induces the production of inflammatory cytokines in both cancer and prostate epithelial cells. Furthermore, ACM promoted proliferation in androgen receptor (AR)-negative PC3 cells, CD44+ PC3 PCSCs, and nonmalignant RWPE cells, without affecting AR-positive LNCaP cells. In addition, ACM-enhanced invasion and migration potential in both PC3 and CD44+ PC3 cells. Western blot analysis indicated the involvement of NF-kappa B and AKT pathways in ACM-induced proliferation in PC3 cells and NF-kappa B in PCSCs. In ACM-treated PC3 cells, E-cadherin was downregulated, while N-cadherin, Snail, vimentin, fibronectin, and Twist were upregulated, suggesting ACM-induced invasion via classical epithelial-to-mesenchymal transition (EMT) pathways. In response to ACM, PCSCs exhibited increased expression of E-cadherin, Snail, and vimentin, which are partial EMT markers promoting stemness and resistance to apoptosis. In addition, increased expressions of Nanog, Oct3/4, survivin, and Bcl-2 were observed. Although the molecules we studied have diverse effects on cellular regulation, our data emphasize obesity's multifaceted role in promoting and aggressing PC, notably affecting PCSC populations. The link between cancer and obesity has been well-established. Excess adipocyte tissue can promote inflammation and hormone imbalances, all of which contribute to cancer development. This study investigated the effectiveness of adipose tissue secretions on different types of prostate cancer (PC) cells, the proliferation and migration of normal prostate epithelial cells. The findings revealed that adipose tissue contributed to the proliferation, invasion, and migration of PC and cancer stem cells. In addition, it showed a potential relationship between adipose tissue and the proliferation of normal prostate epithelial cells.en_US
dc.description.sponsorshipTrakya University Research Project Foundation; Edirne, Turkey; [2020-101]en_US
dc.description.sponsorshipThis study was funded by the Trakya University Research Project Foundation (Project no: 2020-101), Edirne, Turkey.en_US
dc.identifier.doi10.1002/cbf.3979
dc.identifier.issn0263-6484
dc.identifier.issn1099-0844
dc.identifier.issue2en_US
dc.identifier.pmid38481004en_US
dc.identifier.scopus2-s2.0-85187725809en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/cbf.3979
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18103
dc.identifier.volume42en_US
dc.identifier.wosWOS:001184388800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofCell Biochemistry And Functionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipocyteen_US
dc.subjectInflammationen_US
dc.subjectInvasionen_US
dc.subjectMigrationen_US
dc.subjectObesityen_US
dc.subjectProstate Canceren_US
dc.subjectStem Cellen_US
dc.subjectNf-Kappa-Ben_US
dc.subjectNeuroendocrineen_US
dc.subjectAdipocytesen_US
dc.subjectIncreasesen_US
dc.subjectSurvivalen_US
dc.subjectEfficacyen_US
dc.subjectIl-6en_US
dc.titleMultifaceted impact of adipose conditioned media: Obesity-driven promotion of prostate cancer and cancer stem cell dynamicsen_US
dc.typeArticleen_US

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