Multifaceted impact of adipose conditioned media: Obesity-driven promotion of prostate cancer and cancer stem cell dynamics
Küçük Resim Yok
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Obesity is an established risk factor for the development and progression of prostate cancer (PC). This study used adipose conditioned media (ACM) from differentiated adipocytes to assess its effect on PC development and aggressiveness. Due to limited research on ACM's impact on isolated PC stem cells (PCSCs), we also examined CD44+ PCSCs. ACM notably boosted interleukin-1 beta (IL-1 beta), IL-6, and IL-8 production in normal prostate epithelial cells and LNCaP cells. It also increased IL-6 and IL-8 production in PC3 and CD44+ LNCaP cells, and IL-1 beta and IL-6 production in CD44+ PC3 cells. This indicates that ACM induces the production of inflammatory cytokines in both cancer and prostate epithelial cells. Furthermore, ACM promoted proliferation in androgen receptor (AR)-negative PC3 cells, CD44+ PC3 PCSCs, and nonmalignant RWPE cells, without affecting AR-positive LNCaP cells. In addition, ACM-enhanced invasion and migration potential in both PC3 and CD44+ PC3 cells. Western blot analysis indicated the involvement of NF-kappa B and AKT pathways in ACM-induced proliferation in PC3 cells and NF-kappa B in PCSCs. In ACM-treated PC3 cells, E-cadherin was downregulated, while N-cadherin, Snail, vimentin, fibronectin, and Twist were upregulated, suggesting ACM-induced invasion via classical epithelial-to-mesenchymal transition (EMT) pathways. In response to ACM, PCSCs exhibited increased expression of E-cadherin, Snail, and vimentin, which are partial EMT markers promoting stemness and resistance to apoptosis. In addition, increased expressions of Nanog, Oct3/4, survivin, and Bcl-2 were observed. Although the molecules we studied have diverse effects on cellular regulation, our data emphasize obesity's multifaceted role in promoting and aggressing PC, notably affecting PCSC populations. The link between cancer and obesity has been well-established. Excess adipocyte tissue can promote inflammation and hormone imbalances, all of which contribute to cancer development. This study investigated the effectiveness of adipose tissue secretions on different types of prostate cancer (PC) cells, the proliferation and migration of normal prostate epithelial cells. The findings revealed that adipose tissue contributed to the proliferation, invasion, and migration of PC and cancer stem cells. In addition, it showed a potential relationship between adipose tissue and the proliferation of normal prostate epithelial cells.
Açıklama
Anahtar Kelimeler
Adipocyte, Inflammation, Invasion, Migration, Obesity, Prostate Cancer, Stem Cell, Nf-Kappa-B, Neuroendocrine, Adipocytes, Increases, Survival, Efficacy, Il-6
Kaynak
Cell Biochemistry And Function
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
42
Sayı
2
