Inhibition of iNOS with S-methylisothiourea was impaired in wound heating in caustic esophageal burn
Küçük Resim Yok
Tarih
2005
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ireland Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: Stricture formation is a late complication of caustic esophageal burn, which is a common problem in childhood. For this reason, this experimental study was designed to observe the possible effect of nitric oxide on heating and fibrosis formation in caustic esophageal burns. Materials and methods: The rats were divided into five groups. Group A (n = 12) received sham burn and treatment with saline injection. Group B (n = 34) received caustic burn. Rats in group C (n = 31), were given water supplement with 10 g/L L-arginine that was started 24 In preoperatively and continued until postoperative day 4. In group D (n = 21), S-methylisothiourea (SMT, specific inducible nitric oxide synthase (iNOS) inhibitor), was injected at a dose of 3 mg/kg i.p. at 30 min before caustic burn, and similar dose was reinjected immediatety after caustic burn. SMT 6 mg/kg/day injections continued for 4 days Long. In group E (n = 22), N omega-nitro-L-arginine (L-NNA, nonspecific nitric oxide synthase (NOS) inhibitor) was injected at a dose of 15 mg/kg i.p. at 30 min before caustic burn, and similar dose was reinjected immediately after caustic burn. L-NNA 30 mg/kg/day continues for 4 days. Results: Dead rates were significantly higher in group Ethan in groups A-D. The mean hydroxyproline levels in esophageal. tissue were significantly lower in groups A and B than in group D. Histopathologically, tissue damage scores in the esophageal tissue were higher in group D than in groups A-C. Conclusions: Inhibition of iNOS with SMT was impaired in wound heating due to caustic esophageal burn and provoked collagen accumulation at a later period. Those effects may due to inhibition of antioxidant, immunomodulatory and antifibrotic effects of NO. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
Caustic Esophageal Burn, Nitric Oxide, S-Methlisothiourea, Inducible Nitric Oxide Synthase, Wound Healing, Nitric-Oxide Synthase, Nf-Kappa-B, Epidermal Growth-Factor, Intestinal Inflammation, Collagen-Synthesis, Antioxidant Therapy, Endothelial-Cells, Deficient Mice, Injury, Rats
Kaynak
International Journal Of Pediatric Otorhinolaryngology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
69
Sayı
4
