In vitro hepatotoxicity evaluation of methotrexate-loaded niosome formulation: fabrication, characterization and cell culture studies

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dc.authoridTurker, Nebiye Pelin/0000-0001-6060-3557
dc.authoridOltulu, Cagatay/0000-0002-6051-3479
dc.authorwosidErgin, Ahmet Dogan/AAO-1876-2021
dc.authorwosidTurker, Nebiye Pelin/AAH-3197-2021
dc.authorwosidOltulu, Cagatay/V-1823-2018
dc.contributor.authorErgin, Ahmet Dogan
dc.contributor.authorOltulu, Cagatay
dc.contributor.authorTurker, Nebiye Pelin
dc.contributor.authorDemirbolat, Gulen Melike
dc.date.accessioned2024-06-12T11:08:26Z
dc.date.available2024-06-12T11:08:26Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground/aim: Methotrexate (MTX) is a folic acid antagonist that is widely used to treat osteosarcoma, leukemia, breast cancer, and autoimmune and inflammatory diseases. The most important concerns with MTX are its poor solubility and high toxicity, particularly in liver cells. To enhance its solubility and to minimize its toxicity, we encapsulated MTX in niosomes and investigated its hepatotoxicity mechanisms using genetic biomarkers. Materials and methods: Niosomes were successfully prepared using a modified thin film method, and the prepared monodisperse smallsized formulation was subsequently characterized. In vitro cytotoxicity studies were performed both in hepatocarcinoma (HEP3G) and healthy liver (AML12) cell lines. Specifically, immunofluorescence assay and evaluation of the expression levels of apoptotic, antioxidant, heat shock protein, and oxidative stress genes were performed. Results: The formulation had a particle size of 117.1 +/- 33 nm, a surface charge of -38.41 +/- 0.7 mV, and an encapsulation efficiency of 59.7% +/- 2.3%. The results showed that the niosomal formulation exhibited significantly higher cytotoxic effects in HEP3G than in AML12. The immunofluorescence and genetic analyses showed that the increased cytotoxicity of niosomes resulted mainly from oxidative stress and slight apoptosis. Conclusion: These results demonstrated that niosomal drug delivery systems could be a new potential formulation for minimizing MTX-related hepatotoxicity.en_US
dc.identifier.doi10.55730/1300-0144.5651
dc.identifier.endpage882en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue4en_US
dc.identifier.pmid38031943en_US
dc.identifier.scopus2-s2.0-85169301106en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage872en_US
dc.identifier.trdizinid1194018en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5651
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1194018
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22434
dc.identifier.volume53en_US
dc.identifier.wosWOS:001166710900004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMethotrexateen_US
dc.subjectNiosomesen_US
dc.subjectHepatotoxicityen_US
dc.subjectOxidative Stressen_US
dc.subjectApoptosisen_US
dc.subjectInduced Apoptosisen_US
dc.subjectDeliveryen_US
dc.titleIn vitro hepatotoxicity evaluation of methotrexate-loaded niosome formulation: fabrication, characterization and cell culture studiesen_US
dc.typeArticleen_US

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