Evaluation of microRNA responses in ARPE-19 cells against the oxidative stress

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dc.authorwosidAyaz, Lokman/K-6716-2013
dc.contributor.authorAyaz, Lokman
dc.contributor.authorDinc, Erdem
dc.date.accessioned2024-06-12T11:11:54Z
dc.date.available2024-06-12T11:11:54Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose: This study aimed to determine microRNA (miRNA) expression profile of human retinal pigment epithelium cell (ARPE-19) against the oxidative stress induced by hydrogen peroxide (H2O2).Methods: ARPE-19 cells were incubated with different concentrations of H2O2 (200, 600 and 800M) for 18h, and then cell viability, vascular endothelial growth factor levels and total oxidant status were evaluated. Expressions of 1152 miRNA were determined by quantitative real-time PCR in each group.Results: Expressions of 90 miRNA were significantly changed in the ARPE-19 cells incubated with H2O2 compared to control group. However, miR-143-3p was only found to be expressed in groups incubated with H2O2. While 24 miRNA (hsa-miR-200c-3p, miR-192-5p, miR-194-5p, miR-141-3p, miR-658, miR-18b-5p, miR-486-5p, miR-525-3p, miR-493-3p, miR-518d-3p, miR-29b-1-5p, miR-675-3p, miR-1238-3p, miR-195-3p, miR-1539, miR-490-5p, miR-3200-5p, miR-1273d, miR-130a-5p, miR-30b-5p, miR-1247-5p, miR-1910-5p, miR27a-5p and miR-200b-3p) upregulated due to the increased dose of H2O2, nine miRNA (hsa-miR-96-5p, miR-33a-5p, miR-345-5p, miR-106b-3p, miR-1285-3p, miR-23b-5p, miR-27b-5p, miR-103a-3p and miR-4289) were also found to be downregulated.Conclusion: This study suggests that oxidative stress may be an important factor on expression of miRNAs in ARPE-19 cells. These miRNAs may have a role in the pathogenesis of age-related macular degeneration related to oxidative stress. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S790]en_US
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) [Grant No. 114S790].en_US
dc.identifier.doi10.1080/15569527.2017.1355314
dc.identifier.endpage126en_US
dc.identifier.issn1556-9527
dc.identifier.issn1556-9535
dc.identifier.issue2en_US
dc.identifier.pmid28707489en_US
dc.identifier.scopus2-s2.0-85026524051en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage121en_US
dc.identifier.urihttps://doi.org/10.1080/15569527.2017.1355314
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22974
dc.identifier.volume37en_US
dc.identifier.wosWOS:000429276400003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofCutaneous And Ocular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOxidative Stressen_US
dc.subjectMirnaen_US
dc.subjectMicrornaen_US
dc.subjectARPE-19 Cellsen_US
dc.subjectAge-Related Macular Degenerationen_US
dc.subjectAMDen_US
dc.subjectDiabetic-Retinopathyen_US
dc.subjectExpressionen_US
dc.subjectInflammationen_US
dc.titleEvaluation of microRNA responses in ARPE-19 cells against the oxidative stressen_US
dc.typeArticleen_US

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