Evaluation of microRNA responses in ARPE-19 cells against the oxidative stress
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: This study aimed to determine microRNA (miRNA) expression profile of human retinal pigment epithelium cell (ARPE-19) against the oxidative stress induced by hydrogen peroxide (H2O2).Methods: ARPE-19 cells were incubated with different concentrations of H2O2 (200, 600 and 800M) for 18h, and then cell viability, vascular endothelial growth factor levels and total oxidant status were evaluated. Expressions of 1152 miRNA were determined by quantitative real-time PCR in each group.Results: Expressions of 90 miRNA were significantly changed in the ARPE-19 cells incubated with H2O2 compared to control group. However, miR-143-3p was only found to be expressed in groups incubated with H2O2. While 24 miRNA (hsa-miR-200c-3p, miR-192-5p, miR-194-5p, miR-141-3p, miR-658, miR-18b-5p, miR-486-5p, miR-525-3p, miR-493-3p, miR-518d-3p, miR-29b-1-5p, miR-675-3p, miR-1238-3p, miR-195-3p, miR-1539, miR-490-5p, miR-3200-5p, miR-1273d, miR-130a-5p, miR-30b-5p, miR-1247-5p, miR-1910-5p, miR27a-5p and miR-200b-3p) upregulated due to the increased dose of H2O2, nine miRNA (hsa-miR-96-5p, miR-33a-5p, miR-345-5p, miR-106b-3p, miR-1285-3p, miR-23b-5p, miR-27b-5p, miR-103a-3p and miR-4289) were also found to be downregulated.Conclusion: This study suggests that oxidative stress may be an important factor on expression of miRNAs in ARPE-19 cells. These miRNAs may have a role in the pathogenesis of age-related macular degeneration related to oxidative stress. However, this relationship needs to be examined in new studies by evaluation of pathways and target genes.
Açıklama
Anahtar Kelimeler
Oxidative Stress, Mirna, Microrna, ARPE-19 Cells, Age-Related Macular Degeneration, AMD, Diabetic-Retinopathy, Expression, Inflammation
Kaynak
Cutaneous And Ocular Toxicology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
37
Sayı
2
