Investigation of Carbapenemase Genes and Clonal Relationship in Carbapenem Resistant Klebsiella pneumoniae Strains

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dc.authoridVahaboglu, H/0000-0001-8217-1767
dc.authoridcaskurlu, hulya/0000-0002-6760-2052
dc.authoridKocoglu, Mucahide Esra/0000-0002-2860-1794
dc.authorwosidVahaboglu, H/HHZ-1169-2022
dc.authorwosidcaskurlu, hulya/ABI-3919-2020
dc.contributor.authorSamasti, Mustafa
dc.contributor.authorKocoglu, Mucahide Esra
dc.contributor.authorDavarci, Ismail
dc.contributor.authorVahaboglu, Haluk
dc.contributor.authorCaskurlu, Hulya
dc.date.accessioned2024-06-12T10:55:41Z
dc.date.available2024-06-12T10:55:41Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: Resistant Gram-negative bacteria isolated from health-related infections are a worldwide problem. Increasing Frequency of infections particularly caused by Enterobacteriaceae producing expanded spectrum beta lactamase, leads to the use of more carbapenem group antibiotics which, in turn, leads to bacterial resistance. In this study, we aimed to evaluate carbapenem resistance in Klebsiella pneumaniae (K. pneumoniae) isolates, the mechanisms causing this resistance and the clonal relationship between these isolates. Methods: Ninety-one K. pneurnolliae strains isolated from clinical samples obtained in our laboratory were included to the study. The identification of the bacteria was performed with Matriks assisted laser desorption ionization time of flight mass spectrometry (bioMerieux, Marcy-I'Etoile, France) and antimicrobial susceptibility with VITEK-2 (bioMerieux), and the carhapenem resistance was confirmed by ertapenem E-test (bioMerieux). Reverse transcription polymerise chain reaction method was used for the investigation of genes causing carbapenemase production (bla(OXA-)(48), bla(NDM-1), bla(KPC), bla(IMP), bla(VIM-1)). The clonal relationship between isolates was investigated by pulsed-field jel elektroforez. Results: In carbapenem resistant isolates, bla(OXA-)(48) positivity was found to be 55 % , bla(NDM-1) positivity 37.4%, bla(KPC) and bla(VIM-1) positivity 1.1%. A total of 10 isolates was identified with different resistance genes. In 73 of the isolates included in the study, the clonal relationship was examined, and 16 different groups were identified. Twenty isolates were not clonally associated with any other isolates. The most common resistance mechanism causing the carbapenem resistance was bia(OXA-48) gene that is known to be endemic in Turkey. Conclusion: As a result, the carbapenem resistance that we found as 3.13% in our study is similar to the rates obtained in other studies performed in our country which indicates that this resistance is not at a high level yet in our country. However, the ability of carbapenem resistance genes to spread between strains can he a major problem in the near future. Molecular methods arc gold standard in carbapenemase detection, but because of having high cost they can not be used in laboratories routinely. Modified Hodge test or carbapenemase inactivation test are alternative tests with low costs that can be used in the determination of carbapenemase.en_US
dc.identifier.doi10.14235/bas.galenos.2018.2675
dc.identifier.endpage190en_US
dc.identifier.issn2148-2373
dc.identifier.issue3en_US
dc.identifier.startpage186en_US
dc.identifier.trdizinid324510en_US
dc.identifier.urihttps://doi.org/10.14235/bas.galenos.2018.2675
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/324510
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19504
dc.identifier.volume7en_US
dc.identifier.wosWOS:000499482100003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherBezmialem Vakif Univen_US
dc.relation.ispartofBezmialem Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbapenemen_US
dc.subjectKlebsiella Pneumoniaeen_US
dc.subjectResistance Geneen_US
dc.subjectPulsed-Field Gel Electrophoresisen_US
dc.subjectMetallo-Beta-Lactamaseen_US
dc.subjectEscherichia-Colien_US
dc.subjectEnterobacteriaceaeen_US
dc.subjectSurveillanceen_US
dc.subjectTurkeyen_US
dc.subjectEpidemiologyen_US
dc.subjectIsolateen_US
dc.titleInvestigation of Carbapenemase Genes and Clonal Relationship in Carbapenem Resistant Klebsiella pneumoniae Strainsen_US
dc.typeArticleen_US

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