Determination of Some Properties of Free and Immobilized Urease from Aspergillus fumigatus and Its Application in Urea Assay

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dc.contributor.authorTetiker, Aylin Turksever
dc.contributor.authorErtan, Figen
dc.date.accessioned2024-06-12T11:16:58Z
dc.date.available2024-06-12T11:16:58Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractUrease enzyme was extracted from Apergillus fumigatus and immobilized in calcium alginate beads. The immobilization efficiency was calculated as 82.5 %. Optimum pH and temperature for free and immobilized enzymes were found to be 7.0 and 40 degrees C, respectively. The immobilized urease had a better Km value but, catalytic efficiencies (kcat/Km) were very similar. Immobilized enzyme maintained 44% of its initial activity after 5 repeated use of enzyme. It was found that storage stability of immobilized enzyme was better than that of the free enzyme. Immobilized urease enzyme was used for the determination of urea amounts in animal feed.en_US
dc.description.sponsorshipTrakya University Research Foundation [TUBAP-2013-147]en_US
dc.description.sponsorshipThis research was supported by Trakya University Research Foundation (Project number: TUBAP-2013-147).en_US
dc.identifier.endpage564en_US
dc.identifier.issn0253-5106
dc.identifier.issue3en_US
dc.identifier.startpage558en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24514
dc.identifier.volume38en_US
dc.identifier.wosWOS:000381933700024en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherChem Soc Pakistanen_US
dc.relation.ispartofJournal Of The Chemical Society Of Pakistanen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUreaseen_US
dc.subjectImmobilizationen_US
dc.subjectCalcium Alginateen_US
dc.subjectAspergillus Fumigatusen_US
dc.subjectAnimal Feeden_US
dc.subjectAlpha-Amylaseen_US
dc.subjectAlginateen_US
dc.subjectEnzymeen_US
dc.subjectPurificationen_US
dc.subjectOptimizationen_US
dc.titleDetermination of Some Properties of Free and Immobilized Urease from Aspergillus fumigatus and Its Application in Urea Assayen_US
dc.typeArticleen_US

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