Abiraterone Acetate, in Combination with Apigenin, Attenuates the Survival of Human Castration-Sensitive Prostate Cancer Cells

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dc.authoridSERTTAS, RIZA/0000-0002-7493-0388
dc.authoridAtabey, Ugur Simal/0000-0001-9002-8645
dc.authoridGenc, Fatih/0000-0003-4319-1102
dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.authorwosidSERTTAS, RIZA/AAG-7463-2020
dc.contributor.authorGenc, Fatih
dc.contributor.authorAtabey, Ugur Simal
dc.contributor.authorSerttas, Riza
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T10:50:15Z
dc.date.available2024-06-12T10:50:15Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Abiraterone acetate (AA) is a selective inhibitor of CYP17 alpha-hydroxylase, which is crucial for androgen biosynthesis. Apigenin (Api) is a natural plant-derived flavonoid with potent antiproliferative and anti-migration effects. Objectives: We aimed to investigate the possible role of Api in combination with the androgen receptor inhibitor AA in the treatment of androgen-sensitive human prostate cancer LNCaP cells. Methods: The cells were either exposed to 10 mu M AA, 25 mu M Api, or in combination for 48 hours, then the viability rate was determined by the MTT test, whilst apoptosis and cell cycle phases were assessed by image-based cytometry. The expression of selected mRNA and proteins were evaluated by RT-qPCR and Western blot, respectively. Results: The combination of AA and Api significantly inhibited LNCaP as well as androgen-insensitive PC3 cell survival in a manner more marked than observed with either single treatment. Co-administration of Api with AA triggered apoptosis. This effect was demonstrated by Hoechst staining, and up-regulation of Bax, cytochrome c, caspase -3, and -8 and down-regulation of Bcl-2 expression confirmed the effect. AA and Api each individually arrested the cell cycle in the G1 phase, with dual applications, leading to no further increase in the effect produced. The expression of NF-kappa B p105/p50 and the phosphorylation of AKT markedly decreased after apigenin treatment, with combination treatment leading to a favourable effect in terms of further augmenting the reduction. Conclusion: The co-administration of Api with AA strongly enhanced the efficacy of AA therapy in the treatment of prostate cancer cells. These data suggested that the combination of AA and Api would be a potential chemotherapeutic strategy against prostate cancer.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK, under 2209-A Research Project Support Programme)en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, under 2209-A Research Project Support Programme).en_US
dc.identifier.doi10.2174/1871520622666220426095257
dc.identifier.endpage3156en_US
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue18en_US
dc.identifier.pmid35473536en_US
dc.identifier.scopus2-s2.0-85137997178en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3148en_US
dc.identifier.urihttps://doi.org/10.2174/1871520622666220426095257
dc.identifier.urihttps://hdl.handle.net/20.500.14551/17934
dc.identifier.volume22en_US
dc.identifier.wosWOS:000860484800010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofAnti-Cancer Agents In Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAbiraterone Acetateen_US
dc.subjectApigeninen_US
dc.subjectApoptosisen_US
dc.subjectCastration Sensitive Prostate Canceren_US
dc.subjectLncapen_US
dc.subjectWestern Bloten_US
dc.subjectCycle Arresten_US
dc.subjectKappa-Ben_US
dc.subjectCarcinomaen_US
dc.subjectEnzalutamideen_US
dc.subjectInhibitionen_US
dc.subjectApoptosisen_US
dc.subjectMigrationen_US
dc.subjectPathwayen_US
dc.subjectRisken_US
dc.subjectPrednisoneen_US
dc.titleAbiraterone Acetate, in Combination with Apigenin, Attenuates the Survival of Human Castration-Sensitive Prostate Cancer Cellsen_US
dc.typeArticleen_US

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