Eupatilin attenuates TGF-?2-induced proliferation and epithelial-mesenchymal transition of retinal pigment epithelial cells

Basit Öğe Kaydı
dc.authoridSERTTAS, RIZA/0000-0002-7493-0388
dc.authoridKupeli Cinar, Ayca/0000-0003-4495-9603
dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.authorwosidSERTTAS, RIZA/AAG-7463-2020
dc.contributor.authorCinar, Ayca Kupeli
dc.contributor.authorOzal, S. Altan
dc.contributor.authorSerttas, Riza
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T11:01:32Z
dc.date.available2024-06-12T11:01:32Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPurpose The main characteristic of proliferative vitreoretinopathy (PVR) is migration, adhesion, and epithelial-mesenchymal transition (EMT) of retinal pigment epithelial cells (RPE). Eupatilin is a naturally occurring flavone that has the potential to inhibit cell proliferation and EMT. However, its efficacy on the PVR model induced by transforming growth factor-2 (TGF-beta 2) is unknown. In this study, the potential effect of eupatilin on proliferation and EMT in the treatment of RPE was investigated. Methods Serum starved human RPE cells (ARPE-19) were treated with 10 ng/ml TGF-beta 2 alone or co-treated with 25 mu M eupatilin for 48 h. Quantitative real-time PCR and Western blot analysis were used to assess targets at the mRNA and protein expression level, respectively. Apoptosis and cell cycle progression was assessed by image-based cytometry. The effect of treatment on cell migration was evaluated by wound healing assay. Results Eupatilin inhibited TGF-beta 2-induced RPE cell proliferation via regulating the cell cycle and inducing apoptosis. TGF-beta 2 upregulated mRNA expression of mesenchymal markers fibronectin and vimentin was significantly downregulated by the treatment, while the epithelial markers E-cadherin and occludin expression was upregulated. The therapy significantly suppressed TGF-beta 2 encouraged cell migration through downregulating the expression of transcription factors Twist, Snail, and ZEB1 induced by TGF-beta 2. Furthermore, eupatilin significantly inhibited the expression of MMP-1, -7, and -9, and suppressed NF-kappa B signalling. Conclusion These results suggest that eupatilin could inhibit the proliferation and transformation into fibroblast-like cells of RPE cells; thus the agent may be a potential therapeutic value in treating PVR.en_US
dc.description.sponsorshipTrakya University Scientific Research Projects Foundation [TUBAP 2019-224]en_US
dc.description.sponsorshipThis work was financially supported by the Trakya University Scientific Research Projects Foundation [TUBAP 2019-224].en_US
dc.identifier.doi10.1080/15569527.2021.1902343
dc.identifier.endpage114en_US
dc.identifier.issn1556-9527
dc.identifier.issn1556-9535
dc.identifier.issue2en_US
dc.identifier.pmid33719768en_US
dc.identifier.scopus2-s2.0-85104062216en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage103en_US
dc.identifier.urihttps://doi.org/10.1080/15569527.2021.1902343
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20929
dc.identifier.volume40en_US
dc.identifier.wosWOS:000638229500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofCutaneous And Ocular Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEupatilinen_US
dc.subjectPVRen_US
dc.subjectEpithelial To Mesenchymal Transitionen_US
dc.subjectRetina Pigment Epithelial Cellen_US
dc.subjectTGF-? 2en_US
dc.titleEupatilin attenuates TGF-?2-induced proliferation and epithelial-mesenchymal transition of retinal pigment epithelial cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu