Investigation of Antimicrobial Susceptibilities and Resistance Genes of Campylobacter Isolates from Patients in Edirne, Turkey

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dc.contributor.authorEryildiz, Canan
dc.contributor.authorSakru, Nermin
dc.contributor.authorKuyucuklu, Gulcan
dc.date.accessioned2024-06-12T10:55:31Z
dc.date.available2024-06-12T10:55:31Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: We aimed to determine the susceptibility of Campylobacter isolates obtained from patients to various antimicrobial agents and to investigate some related antimicrobial resistance genes. Methods: Fifty-six Campylobacter isolates obtained from fecal specimens by conventional methods at the Trakya University Health Center for Medical Research and Practice, Department of Medical Microbiology in Edirne, Turkey, from 2017-2017 were included. Antimicrobial susceptibilities were investigated by the gradient strip test method, and species determination was made by multiplex polymerase chain reaction (mPCR). The presence of the erm(B) gene and tet(O) gene was investigated in all isolates by PCR. DNA sequence analysis was performed to detect the presence of mutations in the 23S rRNA positions 2074 and 2075 in five isolates, including two erythromycin resistant isolates. The gyrA gene mutation was investigated by the mismatch amplification mutation assay (MAMA)-PCR. Results: In 54 C. jejuni isolates, resistance to erythromycin was 3.7%; to tetracycline, 59.3%; and to ciprofloxacin, 74.1%. Phenotypically, the tet(O) gene was detected in 33 tetracycline-resistant isolates, but no erm(B) gene was found in any of the Campylobacter isolates. As a result of the DNA sequencing, it was found no mutations in the 23S rRNA gene at the 2074 and 2075 positions. The gyrA mutation was observed in all 41 ciprofloxacin resistant Campylobacter isolates. Conclusion: Among the antimicrobial agents tested, ciprofloxacin had the highest resistance rate, and erythromycin had the lowest. Antimicrobial resistance in Campylobacter increased significantly compared with previously studies in our region as well as in the entire world. Monitoring the resistance to antimicrobial agents used to treat Campylobacter infections is important in determining empiric antimicrobial treatment.en_US
dc.description.sponsorshipTrakya University Scientific Research Fund [TUBAP-2018/305]en_US
dc.description.sponsorshipThis study was financially supported by the Trakya University Scientific Research Fund (TUBAP-2018/305).en_US
dc.identifier.endpage577en_US
dc.identifier.issn2251-6085
dc.identifier.issn2251-6093
dc.identifier.issue3en_US
dc.identifier.pmid35865057en_US
dc.identifier.scopus2-s2.0-85126295859en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage569en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19458
dc.identifier.volume51en_US
dc.identifier.wosWOS:000771593700010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIranian Scientific Society Medical Entomologyen_US
dc.relation.ispartofIranian Journal Of Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCampylobacteren_US
dc.subjectAntimicrobial Resistanceen_US
dc.subjectResistance Genesen_US
dc.subjectAntibiotic-Resistanceen_US
dc.subjectFluoroquinolone Resistanceen_US
dc.subjectErythromycin Resistanceen_US
dc.subjectPcr Assayen_US
dc.subjectJejunien_US
dc.subjectMechanismsen_US
dc.subjectCiprofloxacinen_US
dc.subjectTetracyclineen_US
dc.subjectMutationsen_US
dc.subjectColien_US
dc.titleInvestigation of Antimicrobial Susceptibilities and Resistance Genes of Campylobacter Isolates from Patients in Edirne, Turkeyen_US
dc.typeArticleen_US

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