Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

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dc.authoridSAKRU, NERMIN/0000-0002-1312-7233
dc.authorwosidSAKRU, NERMIN/M-9952-2017
dc.contributor.authorEryildiz, Canan
dc.contributor.authorSakru, Nermin
dc.date.accessioned2024-06-12T11:00:00Z
dc.date.available2024-06-12T11:00:00Z
dc.date.issued2012
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: Echinococcus granulosus is the causative agent of cystic echinococcosis in humans and many domestic animals, and remains an important global health problem. The aim of this study was to genotype E. granulosus isolates obtained from humans and animals in the Thrace Region of Turkey. Material and Methods: A total of 58 isolates were obtained from patients who underwent surgery at several hospitals and from animals at a slaughterhouse in the province of Edirne. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of ribosomal internal transcribed spacer 1 fragments, and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the partial mitochondrial NADH dehydrogenase subunit 1 (ND1) gene, was used to characterize human and animal E. granulosus isolates. To investigate the genetic characteristics of isolates, deoxyribonucleic acid (DNA) sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and ND1 genes was performed. Results: Fifty-eight E. granulosus isolates, including 42 from human, 13 from cattle and 3 from sheep were, analyzed. The results indicated two distinct genotypes: the G1 (sheep strain) and G7 (pig strain) genotypes. The sheep strain was shown to be the most common genotype of E. granulosus affecting humans, sheep and cattle. Among the concatenated partial CO1 and ND1 sequence data, eight haplotypes of Echinococcus species were identified in the present study. Conclusion: This is the first report indicating that the E. granulosus pig strain is present in humans in this region. We suggest that new strategies be designed for E. granulosus control programs in Turkey.en_US
dc.description.sponsorshipTrakya University Scientific Research Fund [TUBAP-2009/61]en_US
dc.description.sponsorshipThis study was financially supported by the Trakya University Scientific Research Fund (TUBAP-2009/61). Ethics approval was granted by the Ethical Committee of Trakya University Medical Faculty.en_US
dc.identifier.doi10.5152/balkanmedj.2012.072
dc.identifier.endpage267en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue3en_US
dc.identifier.pmid25207011en_US
dc.identifier.scopus2-s2.0-84866704302en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage261en_US
dc.identifier.urihttps://doi.org/10.5152/balkanmedj.2012.072
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20665
dc.identifier.volume29en_US
dc.identifier.wosWOS:000315506200007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEchinococcus Granulosusen_US
dc.subjectGenotypeen_US
dc.subjectPCR-RFLPen_US
dc.subjectPCR-SSCPen_US
dc.subjectCO1en_US
dc.subjectND1en_US
dc.subjectEdirneen_US
dc.subjectThrace Regionen_US
dc.subjectTurkeyen_US
dc.subjectGenetic-Characterizationen_US
dc.subjectCystic Echinococcosisen_US
dc.subjectCervid Strainen_US
dc.subjectG1 Genotypeen_US
dc.subjectCattleen_US
dc.subjectDnaen_US
dc.subjectSequenceen_US
dc.subjectIranen_US
dc.subjectIdentificationen_US
dc.subjectSheepen_US
dc.titleMolecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkeyen_US
dc.typeArticleen_US

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