Different responses of apoptotic, inflammatory and heat shock protein gene expression to a single bout of high-intensity interval exercise between physically active and inactive men

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dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridKaya, Oktay/0000-0001-9639-8022
dc.authoridTAYFUR, PINAR/0000-0002-2825-0760
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidKaya, Oktay/JGL-9169-2023
dc.contributor.authorVardar, S. A.
dc.contributor.authorDoganlar, Z. B.
dc.contributor.authorKaya, O.
dc.contributor.authorTayfur, P.
dc.contributor.authorSut, N.
dc.contributor.authorDoganlar, O.
dc.date.accessioned2024-06-12T10:52:27Z
dc.date.available2024-06-12T10:52:27Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractHigh mechanical load of muscles may induce muscular apoptosis on the one hand and adaptation to exercise on the other. This study aimed to explore whether changes of circulatory levels of inflammation, apoptosis and heat shock proteins (HSPs) messenger RNA (mRNA) following single bout of high-intensity interval exercise (HIIE) differs between physically active (PA) and inactive (PI) men. Nine PA and 9 PI (peak oxygen consumption: 2.6 +/- 0.4 vs. 2.0 +/- 0.2 L.min(-1)) healthy men (age: 28.7 +/- 6.3 vs. 30.2 +/- 4.5 years and body mass index: 2.6 +/- 2.1 vs. 23.3 +/- 2.8 kg.m(-2)) performed HIIE, comprising 4 repeats of a Wingate test (load: 0.050 kg.kg(-1) body weight). Blood samples were collected before exercise, 5 min after HIIE, and 24 h after HIIE for measuring mRNA of inflammation markers interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF alpha), apoptosis markers including Bcl-2, Bax, and HSP27, HSP60, HSP70, HSP90 using quantitative real-time PCR analysis. Post-HIIE IL-6, TNF alpha and HSP60 were higher in the PI than the PA group 5 min after exercise (p = 0.003, effect size (ES) = 1.59; p = 0.007, ES = 1.59 and p = 0.027, ES = 1.10 respectively). HSP70 acutely increased only in the PA group (p = 0.024, ES = 1.20). The increase in Bcl-2 (p = 0.047, ES = 1.08) and Bax (p = 0.024, ES = 1.20) levels were higher in the PI group 5 min after HIIE. The present study indicated that the response of inflammatory, apoptosis and HSP gene expressions to HIIE in blood of healthy male volunteers strongly depends on their level of regular physical activity. Novelty: Blood IL-6 and HSP60 mRNA levels following high intensity exercise may indicate metabolic stress. Increased blood HSP70 mRNA in physically active men may show an alternative apoptosis suppression pathway.en_US
dc.description.sponsorshipTrakya University Research Fund [TUBAP-2017-103]en_US
dc.description.sponsorshipWe are grateful to Dr. Ali Alper Uyar for his help to recruit the volunteers and to Aysegul Meryem Merakli for helping in collecting the blood samples in this study. This work was supported by grants from the Trakya University Research Fund TUBAP-2017-103. The sponsor had no role in the design, analysis or writing of this article.en_US
dc.identifier.doi10.1139/apnm-2020-0783
dc.identifier.endpage752en_US
dc.identifier.issn1715-5312
dc.identifier.issn1715-5320
dc.identifier.issue7en_US
dc.identifier.pmid33439763en_US
dc.identifier.scopus2-s2.0-85109524340en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage743en_US
dc.identifier.urihttps://doi.org/10.1139/apnm-2020-0783
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18694
dc.identifier.volume46en_US
dc.identifier.wosWOS:000671903000007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherCanadian Science Publishingen_US
dc.relation.ispartofApplied Physiology Nutrition And Metabolismen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectHeat Shock Proteinen_US
dc.subjectHigh-Intensity Interval Exerciseen_US
dc.subjectInflammationen_US
dc.subjectIL-6en_US
dc.subjectTNF Alphaen_US
dc.subjectSkeletal-Muscleen_US
dc.subjectHsp70en_US
dc.subjectInterleukin-6en_US
dc.subjectReliabilityen_US
dc.subjectMetabolismen_US
dc.subjectChaperonesen_US
dc.subjectLeukocytesen_US
dc.subjectIncreaseen_US
dc.subjectMarkersen_US
dc.subjectBlooden_US
dc.titleDifferent responses of apoptotic, inflammatory and heat shock protein gene expression to a single bout of high-intensity interval exercise between physically active and inactive menen_US
dc.typeArticleen_US

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