Production and Determination of Some Biochemical Properties of Protease Enzyme by Trichothecium roseum Under Solid State Fermentation

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dc.authoridErtan, Figen/0000-0002-7882-3993
dc.contributor.authorOzkan, Ebru
dc.contributor.authorErtan, Figen
dc.date.accessioned2024-06-12T11:13:04Z
dc.date.available2024-06-12T11:13:04Z
dc.date.issued2012
dc.departmentTrakya Üniversitesien_US
dc.description.abstractSome fungal species were searched in terms of protease production by using casein degrading protease screening methods and it was found that Trichothecium roseum Penicillium piceum, Penicillium chrysogenum and Aspergillus wentii had the highest protease activity. Solid state fermentation was carried out by using wheat bran as substrate and it was determined that T. roseum had the highest protease activity. A number of culture conditions for protease production by T. roseum under SSF were investigated. Maximal protease production was obtained with initial moisture content of 85 % (w/v), an inoculum level of 2 mL (1x10(6) spore/mL) when incubated at 30 degrees C for 7 days. Maximum activity of protease with casein as a substrate was observed after 10 minutes at 40 degrees C temperature and at pH 6.5. The K-m and V-max values of protease for casein as substrate were calculated to be 6.06 (mg/mL) and 121.95 (U/mL), respectively.en_US
dc.identifier.endpage6912en_US
dc.identifier.issn1224-5984
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84858329465en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage6903en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23389
dc.identifier.volume17en_US
dc.identifier.wosWOS:000301704600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherArs Docendien_US
dc.relation.ispartofRomanian Biotechnological Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProteaseen_US
dc.subjectSolid State Fermentation (SSF)en_US
dc.subjectTrichothecium Roseumen_US
dc.subjectAlkaline Proteaseen_US
dc.subjectThermophilic Fungusen_US
dc.subjectProcess Parametersen_US
dc.subjectPenicillium Spen_US
dc.subjectOptimizationen_US
dc.subjectMetalloproteaseen_US
dc.subjectPurificationen_US
dc.subjectSubstrateen_US
dc.titleProduction and Determination of Some Biochemical Properties of Protease Enzyme by Trichothecium roseum Under Solid State Fermentationen_US
dc.typeArticleen_US

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