Potent Suppression of Prostate Cancer Cell Growth and Eradication of Cancer Stem Cells by CD44-targeted Nanoliposome-quercetin Nanoparticles

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dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.contributor.authorTurkekul, Kader
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T11:13:20Z
dc.date.available2024-06-12T11:13:20Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe bioavailability of quercetin, a natural compound, is hindered by low solubility, limited absorption, and restricted systemic availability. Therefore, encapsulating it in biocompatible nanoparticles presents a promising solution. This study aimed to target prostate cancer stem cells (CSCs) overexpressing CD44+ receptors as well as cancer cells, employing quercetin-loaded hyaluronic acid-modified nanoliposomes (LP-Quer-HA). Synthesized via a green ethanol injection method, these nanoliposomes had an average diameter of 134 nm and an impressive loading efficiency of 96.9%. Human prostate cancer cells were treated with either 10 mu M of free quercetin or the same concentration delivered by LP-Quer-HA for 72 hours. Free quercetin reduced androgen-resistant PC3 cell viability by 16%, while LP-Quer-HA significantly increased cell death to 60%. It induced apoptosis, upregulating cytochrome c, Bax, caspases 3 and 8, and downregulating survivin and Bcl-2 expression. Compared to free quercetin, LP-Quer-HA upregulated E-cadherin expression while inhibiting cell migration and reducing the expression of fibronectin, N-cadherin, and MMP9. Treatment of PC3 cell tumor spheroids with LP-Quer-HA decreased the number of CD44 cells and expression of CD44, Oct3/4 and Wnt. Moreover, LP-Quer-HA inhibited p-ERK expression while increasing p38/MAPK and NF-.B protein expression. In androgen-sensitive LNCaP cells, LP-Quer-HA efficacy was notable, reducing cell viability from 10% to 52% compared to free quercetin. Utilizing HA-modified nanoliposomes as a quercetin delivery system enhanced its potency at lower concentrations, reducing the CD44+ cell population and effectively impeding prostate cancer cell proliferation and migration. These findings underscore the potential of quercetin-loaded cationic nanoliposomes as a robust therapeutic approach.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Trakya University [2017-137]en_US
dc.description.sponsorshipThis study was funded by Scientific Research Projects Coordination Unit of Trakya University (Project number: 2017-137).en_US
dc.identifier.doi10.15430/JCP.2023.28.4.160
dc.identifier.endpage174en_US
dc.identifier.issn2288-3649
dc.identifier.issn2288-3657
dc.identifier.issue4en_US
dc.identifier.pmid38205358en_US
dc.identifier.startpage160en_US
dc.identifier.urihttps://doi.org/10.15430/JCP.2023.28.4.160
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23495
dc.identifier.volume28en_US
dc.identifier.wosWOS:001164469400004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Soc Cancer Preventionen_US
dc.relation.ispartofJournal Of Cancer Preventionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectQuercetinen_US
dc.subjectLiposomesen_US
dc.subjectNanoparticlesen_US
dc.subjectNeoplasmsen_US
dc.subjectProstate Canceren_US
dc.subjectTherapeutic Useen_US
dc.subjectNf-Kappa-Ben_US
dc.subjectMigrationen_US
dc.subjectApigeninen_US
dc.subjectCd44en_US
dc.subjectInductionen_US
dc.subjectLiposomesen_US
dc.subjectSurvivalen_US
dc.subjectReleaseen_US
dc.subjectTargeten_US
dc.subjectDeathen_US
dc.titlePotent Suppression of Prostate Cancer Cell Growth and Eradication of Cancer Stem Cells by CD44-targeted Nanoliposome-quercetin Nanoparticlesen_US
dc.typeArticleen_US

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