The Role of Melatonin in Oxidative Stress, DNA Damage, Apoptosis and Angiogenesis in Fetal Eye under Preeclampsia and Melatonin Deficiency Stress

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dc.authoridUZUN, Metehan/0000-0003-1406-5473
dc.authoridOztopuz, Rahime Ozlem/0000-0002-1373-6311
dc.authoridDoganlar, Zeynep Banu/0000-0002-1365-9897
dc.authoridDOĞAN, AYTEN/0000-0003-2525-785X
dc.authoridGüçlü, Hande/0000-0002-3021-0493
dc.authoridDoganlar, Oguzhan/0000-0003-2654-7269
dc.authorwosidUZUN, Metehan/JWR-0343-2024
dc.authorwosidOztopuz, Rahime Ozlem/AFK-7508-2022
dc.authorwosidDoganlar, Zeynep Banu/B-4845-2008
dc.authorwosidDOĞAN, AYTEN/ABA-6754-2020
dc.authorwosidDoğanlar, Oğuzhan/A-2315-2019
dc.authorwosidGüçlü, Hande/AAW-9756-2020
dc.contributor.authorDoganlar, Zeynep Banu
dc.contributor.authorGuclu, Hande
dc.contributor.authorOztopuz, Ozlem
dc.contributor.authorTurkon, Hakan
dc.contributor.authorDogan, Ayten
dc.contributor.authorUzun, Metehan
dc.contributor.authorDoganlar, Oguzhan
dc.date.accessioned2024-06-12T11:01:52Z
dc.date.available2024-06-12T11:01:52Z
dc.date.issued2019
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAim: The aim of this study was to investigate the possible mechanisms of ocular damage induced by pinealectomy (PNX) and preeclampsia (PE), and to determine the cellular and molecular effects of melatonin treatment on oxidative stress, DNA damage, molecular chaperone responses, induction of apoptosis and angiogenesis in the fetal eye of both PNX and PNX+PE animals. Material and Methods: We analysed therapeutic potential of melatonin on fetal eye damage in PNX and PNX+PE animals using Malondialdehyde (MDA), Random Amplified Polymorphic DNA (RAPD), qRT-PCR and Western blot assays. Results: Our study presents three preliminary findings: (a) in fetal eye tissues, PNX and PNX+PE significantly induce oxidative damage to both DNA and protein contents, leading to a dramatic increase in caspase-dependent apoptotic signalling in both mitochondrial and death receptor pathways; (b) the same conditions trigger hypoxia biomarkers in addition to significant overexpression of HIF1-alpha, HIF1-beta, MMP9 and VEGF genes in the fetal eye; (c) finally, melatonin regulates not only the expression of genes encoding antioxidant enzymes and increase in DNA damage as well as lipid peroxidation but also limits programmed cell death processes in the fetal eye of PNX and PNX+PE animals . Furthermore, melatonin can relatively modulate genes in the HIF1 family, TNF-alpha and VEGF, thus acting as a direct anti-angiogenic molecule. In conclusion, both PNX and PNX+PE induce ocular damage at both cellular and molecular levels in fetal eye tissue of rats. Conclusion: Our results clearly indicate the potential of melatonin as a preventative therapeutic intervention for fetal ocular damage triggered by both PNX and PNX+PE.en_US
dc.identifier.doi10.1080/02713683.2019.1619778
dc.identifier.endpage1169en_US
dc.identifier.issn0271-3683
dc.identifier.issn1460-2202
dc.identifier.issue10en_US
dc.identifier.pmid31090463en_US
dc.identifier.scopus2-s2.0-85067507031en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1157en_US
dc.identifier.urihttps://doi.org/10.1080/02713683.2019.1619778
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21064
dc.identifier.volume44en_US
dc.identifier.wosWOS:000474083800001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofCurrent Eye Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFetal Eyeen_US
dc.subjectPinealectomyen_US
dc.subjectPreeclampsiaen_US
dc.subjectOxidative Stressen_US
dc.subjectApoptosisen_US
dc.subjectHypoxiaen_US
dc.subjectMelatonin Therapyen_US
dc.subjectRetinal-Pigment Epitheliumen_US
dc.subjectVitamin-Een_US
dc.subjectProvides Neuroprotectionen_US
dc.subjectGrowth Restrictionen_US
dc.subjectPregnant-Womenen_US
dc.subjectBlood-Pressureen_US
dc.subjectMessenger-Rnaen_US
dc.subjectHeat-Shocken_US
dc.subjectExpressionen_US
dc.subjectPlacentaen_US
dc.titleThe Role of Melatonin in Oxidative Stress, DNA Damage, Apoptosis and Angiogenesis in Fetal Eye under Preeclampsia and Melatonin Deficiency Stressen_US
dc.typeArticleen_US

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