Eupatilin Inhibits the Proliferation and Migration of Prostate Cancer Cells through Modulation of PTEN and NF-?B Signaling

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dc.authoridSERTTAS, RIZA/0000-0002-7493-0388
dc.authoridERDOGAN, SUAT/0000-0002-6823-6293
dc.authoridKoroglu, Cagla/0000-0003-4802-7213
dc.authorwosidSERTTAS, RIZA/AAG-7463-2020
dc.contributor.authorSerttas, Riza
dc.contributor.authorKoroglu, Cagla
dc.contributor.authorErdogan, Suat
dc.date.accessioned2024-06-12T10:52:22Z
dc.date.available2024-06-12T10:52:22Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: Despite advances in the treatment of prostate cancer, side effects and the risks of developing drug resistance require new therapeutic agents. Eupatilin is a secondary metabolite of Artemisia asiatica and has shown potential anti-tumor activity in some cancers, but its potential in prostate cancer treatment has not yet been evaluated. Objective: The aim of the study was to investigate the effectiveness of eupatilin on prostate cancer cell proliferation and migration. Methods: Human prostate cancer PC3 and LNCaP cells were exposed to eupatilin and its efficacy on cell survival was determined by the MTT test. Apoptosis and cell cycle phases were evaluated by an image-based cytometer. Cell migration and invasion were evaluated by wound healing and matrigel migration assays; the expression of mRNA and protein was assessed by RT-qPCR and Western blot, respectively. Results: Eupatilin timeand dose-dependently reduced the viability of prostate cancer cells. Exposure of PC3 cells to 12.5 mu M-50 mu M eupatilin resulted in apoptosis by upregulating the expression of caspase 3, Bax and cytochrome c. Annexin V assessment also confirmed that eupatilin causes apoptosis. The treatment significantly upregulated the mRNA expression of p53, p21, and p27, causing cell cycle arrest in the G(1) phase. Administration of eupatilin inhibited migration and invasion of the cells by downregulating the expression of Twist, Slug and MMP-2,-7. In addition, the agent increased protein expression of tumor suppressor PTEN, while transcription factor NF-kappa B expression was reduced. Conclusion: Eupatilin strongly prevents the proliferation of prostate cancer cells, and suppresses migration and invasion. Due to its therapeutic potential, the clinical use of eupatilin in prostate cancer should also be supported by in vivo studies.en_US
dc.description.sponsorshipTrakya University Research Project Foundation, Turkey [TUBAP: 2018-312]en_US
dc.description.sponsorshipThis study was partly funded by the Trakya University Research Project Foundation (TUBAP: 2018-312), Turkey.en_US
dc.identifier.doi10.2174/1871520620666200811113549
dc.identifier.endpage382en_US
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue3en_US
dc.identifier.pmid32781972en_US
dc.identifier.scopus2-s2.0-85096956896en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage372en_US
dc.identifier.urihttps://doi.org/10.2174/1871520620666200811113549
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18685
dc.identifier.volume21en_US
dc.identifier.wosWOS:000620569800007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofAnti-Cancer Agents In Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectCell Cycleen_US
dc.subjectEupatilinen_US
dc.subjectInvasionen_US
dc.subjectMigrationen_US
dc.subjectMetastasisen_US
dc.subjectProstate Canceren_US
dc.subjectEpithelial-Mesenchymal-Transitionen_US
dc.subjectMatrix Metalloproteinasesen_US
dc.subjectDown-Regulationen_US
dc.subjectCycle Arresten_US
dc.subjectApigeninen_US
dc.subjectSurvivalen_US
dc.subjectInvasionen_US
dc.subjectTumoren_US
dc.subjectActivationen_US
dc.subjectExpressionen_US
dc.titleEupatilin Inhibits the Proliferation and Migration of Prostate Cancer Cells through Modulation of PTEN and NF-?B Signalingen_US
dc.typeArticleen_US

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