Isolation and characterisation of secondary metabolites from Trifolium vesiculosum Savi and their antiproliferative activities

Basit Öğe Kaydı
dc.authoridDemir, Dicle/0000-0001-9257-5765
dc.authorwosidDemir, Dicle/P-6867-2019
dc.contributor.authorCevik, Dicle
dc.contributor.authorAru, Basak
dc.contributor.authorKaragoz, Sanem
dc.contributor.authorGurizi, Niyazi
dc.contributor.authorDemirkiran, Ozlem
dc.date.accessioned2024-06-12T11:16:42Z
dc.date.available2024-06-12T11:16:42Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractOne unreported flavonol namely morin-7-O-methyl ether (1) along with seven known compounds were isolated from the aerial parts of Trifolium vesiculosum Savi which were elucidated by using extensive spectroscopic methods such as 1D and 2D NMR and HR-MS. According to the cell viability assay (MTS) on the purified compounds (1-8), quercetin-3-O-(6''-trans-p-coumaroyl)-& beta;-galactoside (4) revealed remarkable antiproliferative activity most particularly against breast cancer cells (IC50 = 2.90 & PLUSMN; 0.25 & mu;M in HCC1937 and 7.98 & PLUSMN; 0.57 & mu;M in MCF7) while moderate inhibitory activity (IC50 = 17.96 & PLUSMN; 0.51-51.70 & PLUSMN; 2.69 & mu;M) on prostate, colorectal and liver cancer cell viability was observed. Further mechanistic examinations (Annexin V/PI staining, DNA content and detection of reactive oxygen species analyses) showed that compound 4 significantly induced apoptosis, enhanced mitochondrial reactive oxygen species (ROS) accumulation, and caused cell cycle arrest in cancer cells by increasing accumulation of cells at G(0)/G(1) and/or G(2)/M phases of the cell cycle.en_US
dc.identifier.doi10.1080/14786419.2023.2246632
dc.identifier.issn1478-6419
dc.identifier.issn1478-6427
dc.identifier.pmid37583128en_US
dc.identifier.scopus2-s2.0-85168093504en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1080/14786419.2023.2246632
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24410
dc.identifier.wosWOS:001049015700001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofNatural Product Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTrifolium Vesiculosum Savien_US
dc.subjectPhytochemical Isolationen_US
dc.subjectSecondary Metabolitesen_US
dc.subjectAntiproliferative Activityen_US
dc.subjectApoptosisen_US
dc.subjectCell Cycleen_US
dc.subject>en_US
dc.subjectPhenolic-Compoundsen_US
dc.subjectPratense L.en_US
dc.subjectIsoflavonesen_US
dc.subjectFlavonoidsen_US
dc.subjectQuercetinen_US
dc.subjectCoumarinsen_US
dc.subjectSpectraen_US
dc.titleIsolation and characterisation of secondary metabolites from Trifolium vesiculosum Savi and their antiproliferative activitiesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu