The role of endocannabinoid system and TRPV1 receptors in the antidepressant and anxiolytic effects of dipyrone in chronic unpredictable mild stress in mice

Basit Öğe Kaydı
dc.authoridGunduz, Ozgur/0000-0002-2470-3021
dc.authoridÇETİNKAYA, MEHMET/0000-0002-4326-794X;
dc.authorwosidGunduz, Ozgur/A-2351-2016
dc.authorwosidÇETİNKAYA, MEHMET/HTM-7983-2023
dc.authorwosidKaradag, Cetin Hakan/H-4899-2013
dc.authorwosidGÜNDÜZ, Özgür/AAH-8717-2019
dc.contributor.authorTopuz, Ruhan Deniz
dc.contributor.authorCetinkaya, Mehmet Zahid
dc.contributor.authorErumit, Dilsat
dc.contributor.authorAydemir, Kubra Duvan
dc.contributor.authorGunduz, Ozgur
dc.contributor.authorKaradag, Cetin Hakan
dc.contributor.authorUlugol, Ahmet
dc.date.accessioned2024-06-12T11:15:41Z
dc.date.available2024-06-12T11:15:41Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAlthough dipyrone is a widely used analgesic and antipyretic, its mechanism of action is not fully clarified. Recent studies have drawn attention to its central effects and its relationship with the endocannabinoid system. The endocannabinoid system plays important roles in processes such as anxiety, depression, fear, and learningmemory. In this study, we aimed to investigate whether endocannabinoid levels change in the amygdala in chronic unpredictable mild stress model in mice and whether cannabinoid and TRPV1 receptors mediate antidepressant and anxiolytic effects of dipyrone. Mice were submitted to chronic unpredictable mild stress protocol of 6-weeks, then behavioral test were performed. In the first part of the study, dipyrone was injected at doses of 150, 300, and 600 mg/kg (i.p.) during behavioral tests. In the second part, the CB1 antagonist AM 251 (1 mg/kg, i.p.), the CB2 antagonist AM630 (1 mg/kg, i.p.), and the TRPV1 antagonist capsazepine (3 mg/kg, i.p.) were administered alone or in combination with 300 mg/kg dipyrone to observe if these receptors mediate dipyrone effects. Endocannabinoid and N-acylethanolamines levels were measured by LC-MS/MS in amygdala. Our results showed that there were no changes in AEA, 2-AG, PEA, OAE levels in the amygdala in mice exposed to chronic unpredictable mild stress model; dipyrone exerted antidepressant and anxiolytic effects at doses of 300 and 600 mg/kg; its anxiolytic effect appears to be mediated via CB1 receptors, whereas TRPV1 receptors seems to mediate its antidepressant action.en_US
dc.description.sponsorshipTrakya University Research Council [TUBAP 2019/277]en_US
dc.description.sponsorshipThis work was supported by a grant from Trakya University Research Council (TUBAP 2019/277). We thank TUTAGEM for performing LC-MS/MS analysis.en_US
dc.identifier.doi10.1016/j.ejphar.2021.174315
dc.identifier.issn0014-2999
dc.identifier.issn1879-0712
dc.identifier.pmid34270988en_US
dc.identifier.scopus2-s2.0-85110297291en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejphar.2021.174315
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24030
dc.identifier.volume908en_US
dc.identifier.wosWOS:000691345400005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofEuropean Journal Of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStressen_US
dc.subjectDipyroneen_US
dc.subjectEndocannabinoiden_US
dc.subjectAmygdalaen_US
dc.subjectCannabinoid Cb1 Receptorsen_US
dc.subjectRestraint Stressen_US
dc.subjectNucleus-Accumbensen_US
dc.subjectActivationen_US
dc.subjectInhibitionen_US
dc.subjectModelen_US
dc.subjectAcetaminophenen_US
dc.subjectContributesen_US
dc.subjectInvolvementen_US
dc.subjectMetamizolen_US
dc.titleThe role of endocannabinoid system and TRPV1 receptors in the antidepressant and anxiolytic effects of dipyrone in chronic unpredictable mild stress in miceen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu