No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model

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dc.authorwosidBudak, metin/Z-3010-2019
dc.contributor.authorBudak, Metin
dc.contributor.authorKilic, Mahmut Alp
dc.contributor.authorKalkan, Tunaya
dc.contributor.authorTuncel, Handan
dc.date.accessioned2024-06-12T10:58:07Z
dc.date.available2024-06-12T10:58:07Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact beta-catenin dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further research are discussed.en_US
dc.description.sponsorshipIstanbul University Research Fund [10808, BYP- 415/060504, 790-231]en_US
dc.description.sponsorshipAcknowledgement This study was funded by Istanbul University Research Fund (Project No: 10808 and BYP- 415/060504 and) Cerrahpasa Medicine Faculty Ethics Committee Permission no: 790-231)en_US
dc.identifier.doi10.56042/ijeb.v61i12.4370
dc.identifier.endpage945en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issue12en_US
dc.identifier.startpage941en_US
dc.identifier.urihttps://doi.org/10.56042/ijeb.v61i12.4370
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19950
dc.identifier.volume61en_US
dc.identifier.wosWOS:001112537100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal Of Experimental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectColorectal Canceren_US
dc.subjectElectromagnetic Radiationen_US
dc.subjectSingle -Strand Conformation Polymorphism (SSCP)en_US
dc.subjectCanceren_US
dc.subjectExpressionen_US
dc.titleNo mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor modelen_US
dc.typeArticleen_US

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