Widespread detection of PER-1-type extended-spectrum beta-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: a nationwide multicenter study

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dc.authoridLeblebicioglu, Hakan/0000-0002-6033-8543
dc.authoridVahaboglu, H/0000-0001-8217-1767
dc.authoridKaygusuz, Arif/0000-0002-1404-1933
dc.authoridbalık, ismail/0000-0003-3630-8851
dc.authorwosidLeblebicioglu, Hakan/A-3960-2016
dc.authorwosidVahaboglu, H/HHZ-1169-2022
dc.authorwosidaygün, gökhan/IZP-8816-2023
dc.authorwosidKaygusuz, Arif/D-7503-2019
dc.authorwosidaygün, gökhan/D-6627-2019
dc.authorwosidbalık, ismail/AER-4184-2022
dc.contributor.authorVahaboglu, H
dc.contributor.authorOzturk, R
dc.contributor.authorAygun, G
dc.contributor.authorCoskunkan, F
dc.contributor.authorYaman, A
dc.contributor.authorKaygusuz, A
dc.contributor.authorLeblebicioglu, H
dc.date.accessioned2024-06-12T11:19:52Z
dc.date.available2024-06-12T11:19:52Z
dc.date.issued1997
dc.departmentTrakya Üniversitesien_US
dc.description.abstractWe studied the prevalence and molecular epidemiology of PER-1-type beta-lactamases among Acinetobacter, Klebsiella, and Pseudomonas aeruginosa strains isolated over a 3-month period id eight university hospitals from distinct regions of Turkey, A total of 72, 92, and 367 Acinetobacter, Klebsiella, and P. aeruginosa isolates were studied, respectively, The presence of bla(PER) aas determined by the colony hybridization method and later confirmed by isoelectric focusing, We detected PER-1-type beta-lactamases in 46% (33/72) of Acinetobacter strains and in 11% (40/367) of P. aeruginosa strains but not in klebsiella strains. PER-1-type enzyme producers were highly resistant to ceftazidime and gentamicin, intermediately resistant to amikaein, and susceptible or moderately susceptible to imipenem and meropenem, Among PER-1-type-beta-lactamase-positive isolates, five Acinetobacter isolates and six P, aeruginosa isolates from different hospitals were selected for ribosomal DNA fingerprinting with EcoRI and SalI, The EcoRI-digested DNAs were later hybridized with a digoxigenin-labelled PER-1 probe, The ribotypes and the lengths of bla(PER)-carrying fragments were identical in four Acinetobacter strains. A single isolate (Ac3) harbored a PER gene on a different fragment (approximately 4.2 kbp) than the others (approximately 3.4 kbp) and showed a clearly distinguishable ribotype, Ribotypes of P. aeruginosa strains obtained with EcoRI showed three patterns. Similarly, in Pseudomonas strains two different EcoRI fragments harbored bla(PER) (approximately 4.2 kbp in five isolates and 3.4 kbp in one isolate), PER-1-type beta-lactamases appear to be restricted to Turkey, However, their clonal diversity and high prevalence indicate a high spreading potential.en_US
dc.identifier.doi10.1128/AAC.41.10.2265
dc.identifier.endpage2269en_US
dc.identifier.issn0066-4804
dc.identifier.issue10en_US
dc.identifier.pmid9333059en_US
dc.identifier.scopus2-s2.0-0342762055en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2265en_US
dc.identifier.urihttps://doi.org/10.1128/AAC.41.10.2265
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25373
dc.identifier.volume41en_US
dc.identifier.wosWOS:A1997XY74700033en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofAntimicrobial Agents And Chemotherapyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSalmonella-Typhimuriumen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectAntibiotic-Resistanceen_US
dc.subjectOuter-Membraneen_US
dc.subjectPlasmidsen_US
dc.subjectStrainsen_US
dc.subjectCephalosporinsen_US
dc.subjectSusceptibilityen_US
dc.subjectCalcoaceticusen_US
dc.subjectEnterococcusen_US
dc.titleWidespread detection of PER-1-type extended-spectrum beta-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: a nationwide multicenter studyen_US
dc.typeArticleen_US

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