Enhanced mitochondrial co-localization of 8-escin micelle and pancreatic tumor accumulation relation

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dc.authorwosidErgin, Ahmet Dogan/AAO-1876-2021
dc.contributor.authorUner, Burcu
dc.contributor.authorErgin, Ahmet Dogan
dc.date.accessioned2024-06-12T10:50:12Z
dc.date.available2024-06-12T10:50:12Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstract8-escin, a triterpenoid saponin derived from plants, is a valuable natural product that possesses strong antiinflammatory, as well as the potential for anticancer effects. However, its limited water solubility restricts its application. The 8-escin was efficiently loaded into Pluronic F127 micelles using the thin film method to improve its solubility and effectiveness. The formulations underwent various tests, including particle size, encapsulation efficiency, and product yield. Human pluripotent fibroblast and AsPC-1 pancreatic cancer cell lines were subjected to MTT studies. Fluorescence-labeled micelles were used to analyze cellular and mitochondrial uptake, and the results were analyzed using flow cytometry. The micelle formulation was found to be optimal with a particle size of 94.8 +/- 6.35 nm, zeta potential of 43.2 +/- 2.62 mV, PDI of 0.13 +/- 0.01, and encapsulation efficiency of 93.20 +/- 0.09. It demonstrated sustained release in all mediums. The micelle formulation had a stronger impact on cancer cells than healthy cells, but when compared to a marketed anticancer drug, it showed less ROS activity on healthy cells. Overall, the fluorescence-labeled micelle had greater uptake by cellular and mitochondrial systems. Micelles show great potential as a drug delivery system for 8-escin and other similar anticancer candidates that operate through a mitochondria-mediated anticancer mechanism.en_US
dc.identifier.doi10.1016/j.jddst.2023.104994
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.scopus2-s2.0-85173239733en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2023.104994
dc.identifier.urihttps://hdl.handle.net/20.500.14551/17914
dc.identifier.volume89en_US
dc.identifier.wosWOS:001099157500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Drug Delivery Science And Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject8-Escinen_US
dc.subjectMicelleen_US
dc.subjectCanceren_US
dc.subjectCellular Uptakeen_US
dc.subjectMitochondria Targetingen_US
dc.subjectIn-Vitroen_US
dc.subjectPolymeric Micellesen_US
dc.subjectPluronic Micellesen_US
dc.subjectCellular Uptakeen_US
dc.subjectNab-Paclitaxelen_US
dc.subjectEscinen_US
dc.subjectApoptosisen_US
dc.subjectCanceren_US
dc.subjectGemcitabineen_US
dc.subjectCellsen_US
dc.titleEnhanced mitochondrial co-localization of 8-escin micelle and pancreatic tumor accumulation relationen_US
dc.typeArticleen_US

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