Testis iskemi reperfüzyonunda vitamin E ve koenzim Q10'un koruyucu rolü
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Testis torsiyonu, spermatik kordun kendi ekseni etrafında dönmesine bağlı olarak, testis ve eklentilerinin kan akımının engellenmesi sonucu gelişen ürolojik acillerden biridir. Bu çalışmada koenzim Q10 ve vitamin E gibi yağda çözünen, güçlü antioksidan olduğu bilinen iki maddenin testis iskemi reperfüzyon hasarındaki etkilerinin karşılaştırılması amaçlanmıştır.300-350 gr arası ağırlıkta 56 adet Sprague-Dawley soyu yetişkin erkek sıçan gelişigüzel olarak her grupta 8 rat olmak üzere 7 gruba ayrıldı. 1. grup sham grubu olarak belirlendi, 2. grupta iskemi reperfüzyon oluşturuldu, 3. grupta iskemi reperfüzyona ek olarak vitamin E (100mg/kg) uygulandı, 4. grupta iskemi reperfüzyon oluşturulmadan sadece vitamin E uygulandı, 5. grupta iskemi reperfüzyona ek olarak koenzim Q10 (10mg/kg) uygulandı, 6. grupta iskemi reperfüzyon oluşturulmadan sadece koenzim Q10 uygulandı ve 7. grupta iskemi reperfüzyona ek olarak vitamin E ile koenzim Q10 birlikte uygulandı.Testislerin histopatolojik değerlendirmesinde:Johson skorundaki düşüş Grup 1 ile Grup 2 ve Grup 2 ile diğer gruplar (Grup 3, 4, 5, 6 ve 7) karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05). Ayrıca iskemi reperfüzyon yapılan, Grup 3, Grup 5 ve Grup 7'de Johnson skorunda azalma gelişmediği gözlenmiş olup iskemi reperfüzyon yapılmayan Sham, Grup 4 ve Grup 6'ya benzer ve eşit sonuçlar elde edilmiştir.Grade skorundaki artış Grup 1 ile Grup 2, 3, 5 ve 7 karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05). Ayrıca Grup 2 ile Grup 4, 6 ve 7 arasında, Grup 3 ile Grup 4 ve 6 arasında, Grup 4 ile Grup 5 ve 7 arasında, Grup 5 ile Grup 6 ve Grup 6 ile Grup 7 arasındaki değerlerde istatistiksel anlamlı farklar bulundu (p<0,05).Seminifer tübül çaplarındaki azalma Grup 1 ile Grup 2, 3, 5 ve 7 karşılaştırıldığında istatistiksel olarak anlamlı bulundu (p<0,05). Ayrıca Grup 2 ile Grup 3, 4, 5, 6 ve 7 arasında, Grup 3 ile Grup 4, 5, 6 ve 7 arasında, Grup 4 ile Grup 5 ve 7 arasında, Grup 5 ile Grup 6 ve Grup 6 ile Grup 7 arasındaki değerlerde istatistiksel anlamlı farklar elde edildi (p<0,05).Biyokimyasal incelemede, gruplar malondialdehid yönünden karşılaştırıldığında istatistiksel fark bulunmamış (p>0.05) olup, katalaz yönünden karşılaştırıldığında ise Grup 1 ile Grup 2, 3, 4, 5; Grup 2 ile Grup 3, 4, 5, 6; Grup 3 ile Grup 4, 5, 6; Grup 4 ile Grup 7 ve Grup 5 ile Grup 7 arasında istatistiksel anlamlı farklar elde edildi (p<0,05).Pozitron emisyon tomografisi ile incelemede ise Grup 1 ile Grup 3 ve 7, Grup 3 ile Grup 4, Grup 4 ile Grup 6 ve 7 ve Grup 5 ile Grup 7 arasında standart tutulum değerleri açısından istatistiksel anlamlı farklar elde edildi (p<0,05).Çalışmamızda kullandığımız antioksidanların (vitamin E ve koenzim Q10); histopatolojik incelemelerde olumlu ve parallel yönde etki gösterdiği, malondialdehid ve katalaz değerleri incelendiğinde özellikle malondialdehid yönünden beklenilen sonuçların gelişmediği ve bu antioksidanların koruyucu etkinliğinin pozitron emisyon tomografisi ile elde edilen standart tutulum değerleri sonuçlarına da belirgin olarak yansımadığı tespit edildi.Anahtar kelimeler: Testis torsiyonu, ürolojik acil, antioksidan.
Testicular torsion which is as a result of the spermatic cord to rotate on its own axis is an urologic emergency that may result in future infertility problems. The pathologic process of testicular torsion is similar to an ischemia reperfusion injury. The present study was aimed to assess the effects of the preventive effects of coenzyme Q10 and vitamin E which are known as antioxidant defense molecules in a rat model of ischemia/reperfusion injury.A total of 56 male Sprague-Dawley rats (300?350 g) were used for the study. Animals were divided randomly into seven groups (8 animals per group) in the following order: Group I: Sham-operated control group without the application of the torsion. Group 2: ischemia reperfusion injury group Group 3: ischemia reperfusion injury group with vitamin E (100mg/kg). Group 4: without creating any ischemia reperfusion injury group with vitamin E (100mg/kg). Group 5: ischemia reperfusion injury group with coenzyme Q10 (10mg/kg). Group 6: without creating any ischemia reperfusion injury group with coenzyme Q10 (10mg/kg). Group 7: ischemia reperfusion injury group with vitamin E (100mg/kg) and coenzyme Q10 (10mg/kg).As the testicular histological assessment:Amount of decreased in Johnson score were compared among the group 1 and group 2, group 2 and other groups (3, 4, 5, 6, 7) and were statistically significant (p<0.05). Furthermore, there were no decrease observed in Johnson score with Group 3, Group 5 and Group 7 with ischemia reperfusion, moreover Group 1, Group 4 and Group 6, without ischemia reperfusion had similar results were obtained equal.There were statistically significant when increment in grade score were compared between group 1 and groups 2, 3, 5, 7 (p <0.05). Futhermore there were statistically significant (p <0.05) differences between group 4 and groups 4, 6, 7; group 3 and groups 4, 6; group 4 and groups 5, 7; group 5 and group 6; group 6 and group 7 (p <0.05).There were statistically significant when reduction in the diameter of the seminiferous tubule were compared between groups such as group 2 and groups 2, 3, 5, 7; group 3 and groups 4, 5, 6, 7; group 4 and groups 5, 7; group 5 and group 6; and group 6 and group 7 (p<0.05).There were no statistically significant differences compared with the groups in terms of malondialdehide (p>0.05). But there were statistically significant differences compared between the groups such as between Goup 1 and Groups 2, 3, 4, 5; Group 2 and Group 3, 4, 5, 6; Grup 3 and Grup 4, 5, 6; Group 4 and Group 7; and Group 5 and Group 7 in terms of catalase (p<0,05).There were statistically significant when positron emulsion tomography assessment differences were compared between groups such as group 1 and groups 1, 3, 7; group 3 and group 4; group 4 and groups 6, 7; group 5 and group 7; in terms of standart uptake value (p<0.05).The results of this histopathologic study show that coenzyme Q10 and vitamin E administration before the reperfusion period of testicular torsion provides a positive and the parallel direction effect. When malondialdehide and catalase values are examined there is no developed statistically significant results particularly in terms of malondialdehide. Protective efficacy of antioxidants is not reflected in results obtained with positron emulsion tomography in standart uptake value maximum significantly.Key words: Testicular torsion, urological emergency, antioxidants.
Testicular torsion which is as a result of the spermatic cord to rotate on its own axis is an urologic emergency that may result in future infertility problems. The pathologic process of testicular torsion is similar to an ischemia reperfusion injury. The present study was aimed to assess the effects of the preventive effects of coenzyme Q10 and vitamin E which are known as antioxidant defense molecules in a rat model of ischemia/reperfusion injury.A total of 56 male Sprague-Dawley rats (300?350 g) were used for the study. Animals were divided randomly into seven groups (8 animals per group) in the following order: Group I: Sham-operated control group without the application of the torsion. Group 2: ischemia reperfusion injury group Group 3: ischemia reperfusion injury group with vitamin E (100mg/kg). Group 4: without creating any ischemia reperfusion injury group with vitamin E (100mg/kg). Group 5: ischemia reperfusion injury group with coenzyme Q10 (10mg/kg). Group 6: without creating any ischemia reperfusion injury group with coenzyme Q10 (10mg/kg). Group 7: ischemia reperfusion injury group with vitamin E (100mg/kg) and coenzyme Q10 (10mg/kg).As the testicular histological assessment:Amount of decreased in Johnson score were compared among the group 1 and group 2, group 2 and other groups (3, 4, 5, 6, 7) and were statistically significant (p<0.05). Furthermore, there were no decrease observed in Johnson score with Group 3, Group 5 and Group 7 with ischemia reperfusion, moreover Group 1, Group 4 and Group 6, without ischemia reperfusion had similar results were obtained equal.There were statistically significant when increment in grade score were compared between group 1 and groups 2, 3, 5, 7 (p <0.05). Futhermore there were statistically significant (p <0.05) differences between group 4 and groups 4, 6, 7; group 3 and groups 4, 6; group 4 and groups 5, 7; group 5 and group 6; group 6 and group 7 (p <0.05).There were statistically significant when reduction in the diameter of the seminiferous tubule were compared between groups such as group 2 and groups 2, 3, 5, 7; group 3 and groups 4, 5, 6, 7; group 4 and groups 5, 7; group 5 and group 6; and group 6 and group 7 (p<0.05).There were no statistically significant differences compared with the groups in terms of malondialdehide (p>0.05). But there were statistically significant differences compared between the groups such as between Goup 1 and Groups 2, 3, 4, 5; Group 2 and Group 3, 4, 5, 6; Grup 3 and Grup 4, 5, 6; Group 4 and Group 7; and Group 5 and Group 7 in terms of catalase (p<0,05).There were statistically significant when positron emulsion tomography assessment differences were compared between groups such as group 1 and groups 1, 3, 7; group 3 and group 4; group 4 and groups 6, 7; group 5 and group 7; in terms of standart uptake value (p<0.05).The results of this histopathologic study show that coenzyme Q10 and vitamin E administration before the reperfusion period of testicular torsion provides a positive and the parallel direction effect. When malondialdehide and catalase values are examined there is no developed statistically significant results particularly in terms of malondialdehide. Protective efficacy of antioxidants is not reflected in results obtained with positron emulsion tomography in standart uptake value maximum significantly.Key words: Testicular torsion, urological emergency, antioxidants.
Açıklama
Tıpta Uzmanlık
Anahtar Kelimeler
Üroloji, Urology
