Evaluation of the protective effects of hesperetin against cisplatin-induced ototoxicity in a rat animal model

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dc.authoridKARACA, Turan/0000-0002-2500-7781
dc.authoridKara, Medine/0000-0002-4941-7662
dc.authorwosiddemirtaş, selim/JED-6784-2023
dc.authorwosidKARACA, Turan/ABD-6669-2020
dc.authorwosidkara, medine/A-1143-2017
dc.contributor.authorKara, Medine
dc.contributor.authorTurkon, Hakan
dc.contributor.authorKaraca, Turan
dc.contributor.authorGuclu, Oguz
dc.contributor.authorUysal, Sema
dc.contributor.authorTurkyilmaz, Mehmet
dc.contributor.authorDemirtas, Selim
dc.date.accessioned2024-06-12T11:11:49Z
dc.date.available2024-06-12T11:11:49Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjectives: We aimed to investigate the effects of hesperetin as a flavanon both histopathologically and immunohistochemically on cochlear apoptosis in a rat model of cisplatin-induced ototoxicity (CIO). The evaluation of the effects of hesperetin on cisplatin-induced hearing loss was performed using distortion product otoacoustic emission (DPOAE). Methods: Twenty-eight wistar albino rats were used in the current study. The rats were randomly divided into four groups with seven rats in each group. Group C was exposed to a single dose of cisplatin (12 mg/kg) by intraperitoneal injection. Group CH received intraperitoneally cisplatin (12 mg/kg) and hesperetin (20 mg/kg). Group H was exposed to hesperetin (20 mg/kg) intraperitoneally. The sham group (group S) received normal saline (6 cc) intraperitoneally. The measurements of DPOAE and signal-noise ratios (SNR) were performed before the treatment and again on the first and 6 days after administration of the drugs. Rats were sacrificed and cochleae were dissected 10 days after drug administration. The cochlear tissue was assessed in all groups by histopathologic, immunohistochemical and TUNEL assay. In addition, serum oxidative stress markers and antioxidant parameters were analyzed. Results: There was a significant difference between the basal value and the sixth day at frequencies 8.4, 9.6 and 9.96 for group C. We also found a significant difference between the first and sixth day at frequencies 7.2, 8.4, 9.6 and 9.96. On the 6th day, there were significant differences between C and S groups at all frequencies except 2.4. We showed a significant difference between C and H groups at frequencies 4.8, 6.0, 8.4, 9.6 and 9.96. There was also a significant difference between C and CH groups at frequencies 2.4, and 3.6. We found lower levels of oxidants and higher levels of antioxidants in CH group as compared to C group. C group had a significantly greater number of TUNEL-positive cells than did S, H and CH groups. The number of TUNEL-positive cells in CH group was higher than in S and H groups. There was a significant difference between the positive PCNA cells of CH group compared to S and H groups in spiral ganglion and stria vascularis. In addition, there were no positive PCNA cells in C group. Conclusions: Hesperetin may prevent ototoxicity by increased antioxidant enzymes and reduced oxidant parameters and protected against apoptosis resulting from a proliferation of cochlear cells in CIO. (C) 2016 Elsevier Ireland Ltd. All rights reserved.en_US
dc.description.sponsorshipCanakkale Onsekiz Mart University BAP project [TSA-2015-534]en_US
dc.description.sponsorshipThe present study was carried out with financial support of Canakkale Onsekiz Mart University BAP project (Grant No: TSA-2015-534).en_US
dc.identifier.doi10.1016/j.ijporl.2016.03.019
dc.identifier.endpage18en_US
dc.identifier.issn0165-5876
dc.identifier.issn1872-8464
dc.identifier.pmid27240489en_US
dc.identifier.scopus2-s2.0-84961967590en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage12en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijporl.2016.03.019
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22944
dc.identifier.volume85en_US
dc.identifier.wosWOS:000378185000004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofInternational Journal Of Pediatric Otorhinolaryngologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOtotoxicityen_US
dc.subjectCisplatinen_US
dc.subjectHesperetinen_US
dc.subjectApopitosisen_US
dc.subjectAntioxidanten_US
dc.subjectOxidative Stressen_US
dc.subjectMolecular-Mechanismsen_US
dc.subjectCell-Deathen_US
dc.subjectPreventionen_US
dc.subjectAntioxidanten_US
dc.subjectHesperidinen_US
dc.titleEvaluation of the protective effects of hesperetin against cisplatin-induced ototoxicity in a rat animal modelen_US
dc.typeArticleen_US

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