Cross-reacting material 197 (CRM197) affects actin cytoskeleton of endothelial cells

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dc.authoridvarol, basak/0000-0002-0597-4571
dc.authoridHaciosmanoglu Aldogan, Ebru/0000-0001-9559-4515
dc.authoridEdis, Bilge Özerman/0000-0002-3499-0474
dc.authoridunlu, ayhan/0000-0001-6033-7148
dc.authorwosidvarol, basak/AAE-7387-2020
dc.authorwosidHaciosmanoglu Aldogan, Ebru/AAK-3496-2020
dc.authorwosidEdis, Bilge Özerman/K-5032-2012
dc.authorwosidBektaş, Muhammet/AAE-5503-2020
dc.authorwosidunlu, ayhan/Q-1843-2016
dc.contributor.authorEdis, Bilge Ozerman
dc.contributor.authorVarol, Basak
dc.contributor.authorHaciosmanoglu, Ebru
dc.contributor.authorUnlu, Ayhan
dc.contributor.authorBektas, Muhammet
dc.date.accessioned2024-06-12T10:50:29Z
dc.date.available2024-06-12T10:50:29Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractCRM197, cross-reacting material 197, is a mutant of diphtheria toxin (DTx). CRM197 is used in pharmacology as a carrier protein. It has been recently shown that CRM197 causes breakdown in actin filaments. In order to show intracellular localization of CRM197 and visualize cell structure via actin cytoskeleton, endothelial cells were cultured and subjected to CRM197 in vitro. To address the interaction between CRM197 and actin both experimental and theoretical studies were carried out. Colocalization of CRM197 with actin filaments was determined by immunofluorescence microscopy. Following 24-hour incubation, the loss of cell-cell contact between cells was prominent. CRM197 was shown to bind to G-actin by gel filtration chromatography, and this binding was confirmed by Western blot analysis of eluted samples obtained following chromatography. Based on crystal structure, docked model of CRM197-actin complex was generated. Molecular dynamics simulation revealed that Lys42, Cys218, Cys233 of CRM197 interacts with Gly197, Arg62 and Ser60 of G-actin, respectively. CRM197 binding to G-actin, colocalization of CRM197 with actin filament, and actin cytoskeleton rearrangement resulting in the loss of cell-cell contact show that actin comes into sight as target molecule for CRM197.en_US
dc.description.sponsorshipScientific Research Project Coordination Unit of Istanbul University [21270, 51249]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Unit of Istanbul University. Projects number: 21270 and 51249. We are thankful to Gamze Kilic Berkmen for her editing assistance.en_US
dc.identifier.doi10.4149/gpb_2017006
dc.identifier.endpage389en_US
dc.identifier.issn0231-5882
dc.identifier.issn1338-4325
dc.identifier.issue4en_US
dc.identifier.pmid28653650en_US
dc.identifier.scopus2-s2.0-85029897318en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage383en_US
dc.identifier.urihttps://doi.org/10.4149/gpb_2017006
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18011
dc.identifier.volume36en_US
dc.identifier.wosWOS:000412155000003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGeneral Physiol And Biophysicsen_US
dc.relation.ispartofGeneral Physiology And Biophysicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectActin Filamentsen_US
dc.subjectCross-Reacting Material 197en_US
dc.subjectDiphtheria Toxinen_US
dc.subjectEndothelial Cellsen_US
dc.subjectToxin Mutant Crm197en_US
dc.subjectDiphtheria-Toxinen_US
dc.subjectProteinen_US
dc.subjectFilamentsen_US
dc.subjectFragmenten_US
dc.subjectVaccinesen_US
dc.titleCross-reacting material 197 (CRM197) affects actin cytoskeleton of endothelial cellsen_US
dc.typeArticleen_US

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