Characteristics of Pseudomonas aeruginosa isolates from intensive care unit

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dc.contributor.authorOzer, Burcin
dc.contributor.authorTatman-Otkun, Muserref
dc.contributor.authorMemis, Dilek
dc.contributor.authorOtkun, Metin
dc.date.accessioned2024-06-12T11:13:04Z
dc.date.available2024-06-12T11:13:04Z
dc.date.issued2009
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe study looked at the antimicrobial resistance patterns, serotypes, molecular types, metallo beta-lactamase, and chromosomal betalactamase enzymes of P. aeruginosa strains isolated from the patients and the staffs of the intensive care unit. P. aeruginosa isolates from the patients as nosocomial pathogens and from the staffs were evaluated for their susceptibilities to the antimicrobials by the disk diffusion and E-test methods. Metallo beta-lactamase enzymes were investigated by E-test, the inducibility of beta - lactamase enzymes were detected by the disk antagonism test. Serotyping was performed by slide agglutination method. The P. aeruginosa isolates were typed by pulsed field gel electrophoresis. Twenty-five P. aeruginosa strains from the patients and three from the staffs were isolated. Fifteen P. aeruginosa, eleven of which composed of MDR bacteria, were found in serogroup E, 7 strains in G, 4 strains in B, and 1 strain in serogroup A. In all 12 bacteria in the MDR and serogroup E, metallo beta-lactamase enzyme was found to be positive. And in other 15 strains, except the bacterium which could not be serotyped, chromosomal beta-lactamase was found to be positive. The result of the molecular typing showed PFGE A pattern. In conclusion, a pattern in PFGE which included bacteria from MDR and serogroup E, G which was observed in the P. aeruginosa strains which was isolated from the staff's hands and from the 5 patients, and PFGE F pattern were found to be observed the most. Finally, the two different clonal strains were found to be established in the intensive care.en_US
dc.description.sponsorshipTrakya University Scientific Research Project [TUBAP 544]en_US
dc.description.sponsorshipThis study was granted by Trakya University Scientific Research Projects (TUBAP 544).en_US
dc.identifier.doi10.2478/s11536-008-0090-2
dc.identifier.endpage163en_US
dc.identifier.issn1895-1058
dc.identifier.issn1644-3640
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-70349564832en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage156en_US
dc.identifier.urihttps://doi.org/10.2478/s11536-008-0090-2
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23400
dc.identifier.volume4en_US
dc.identifier.wosWOS:000264616600002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherDe Gruyter Poland Sp Z O Oen_US
dc.relation.ispartofCentral European Journal Of Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPseudomonas Aeruginosaen_US
dc.subjectMetallo Beta-Lactamaseen_US
dc.subjectChromosomal Beta-Lactamaseen_US
dc.subjectPulsed Field Gel Electrophoresisen_US
dc.subjectSerotypesen_US
dc.subjectIntensive Care Uniten_US
dc.subjectBeta-Lactamaseen_US
dc.subjectAntimicrobial Susceptibilityen_US
dc.subjectAcinetobacter-Spp.en_US
dc.subjectTobramycinen_US
dc.titleCharacteristics of Pseudomonas aeruginosa isolates from intensive care uniten_US
dc.typeArticleen_US

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