Enzymatic synthesis of prebiotic carbohydrates from lactose: Kinetics and optimization of transgalactosylation activity of ?-galactosidase from Aspergillus oryzae

Basit Öğe Kaydı
dc.authoridGunes, Gurbuz/0000-0002-2948-3785
dc.authoridCinar, Kadir/0000-0001-5488-652X
dc.authorwosidGunes, Gurbuz/I-3575-2014
dc.authorwosidCinar, Kadir/ABF-5591-2021
dc.contributor.authorCinar, Kadir
dc.contributor.authorGunes, Gurbuz
dc.contributor.authorGulec, Haci Ali
dc.date.accessioned2024-06-12T11:18:57Z
dc.date.available2024-06-12T11:18:57Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of the study was to analyze galactooligosaccharide (GOS) synthesis catalyzed by beta-galactosidase from Aspergillus oryzae by applying experimental data to optimization and kinetic parameter estimation methods. The apparent kinetic parameters were determined in varying initial lactose concentrations by using six-step, 11-parameter GOS synthesis mechanism. The results revealed that GOS yield was mainly dependent on initial lactose concentration; on the other hand, all of the reaction parameters affected the required time to achieve maximum GOS yield (TMGY) significantly. The values of 32 degrees Brix, 36.8 degrees C, and 8.32 U/g lactose solution were determined as the optimum reaction experimental set which offers 26.73% GOS yield and 369.82 min TMGY. The increased enzyme-galactose complex dissociation ratio (k(3)/k(-3)) with decreasing initial lactose concentration resulted to low GOS productivity although GOS production/GOS dissociation rate ratio (k(4)/k(-4)) increased with decreasing initial lactose concentration. This result signified that hydrolysis characteristics dominated the reaction medium at low initial lactose concentration. The role of monosaccharide inhibition became important on GOS synthesis for increasing initial lactose concentration. Practical Applications The proposed study deals with investigation of the effects of initial lactose concentration, enzyme concentration, and temperature on enzymatic synthesis of galactoligosaccharide (GOS) from lactose by beta-galactosidase from Aspergillus oryzae (beta gAO) and estimation of apparent kinetic rate constants of the reaction which is important to design of futuristic strategies improving the GOS yield. Low GOS yield is the main concern of the prebiotic food industry due to thermodynamic and kinetic realities of the reaction. Deeper evaluation of the reaction mechanism will contribute valuable data to the partners in the scope of designing more productive engineering methods.en_US
dc.description.sponsorshipFound of Scientific Research Projects of Istanbul Technical University (ITUBAP Project) [MDK-2017-40556]en_US
dc.description.sponsorshipThis research was supported by the Found of Scientific Research Projects of Istanbul Technical University (ITUBAP Project ID: MDK-2017-40556). beta-galactosidase from A. oryzae was kindly donated by Enzyme Development Corporation (EDC) (New York, NY).en_US
dc.identifier.doi10.1111/jfpe.13435
dc.identifier.issn0145-8876
dc.identifier.issn1745-4530
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85084494986en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1111/jfpe.13435
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25014
dc.identifier.volume43en_US
dc.identifier.wosWOS:000530975100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Food Process Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGalactooligosaccharide Productionen_US
dc.subjectOligosaccharidesen_US
dc.subjectHydrolysisen_US
dc.subjectConversionen_US
dc.subjectPolyethyleneimineen_US
dc.subjectImmobilizationen_US
dc.subjectWheyen_US
dc.titleEnzymatic synthesis of prebiotic carbohydrates from lactose: Kinetics and optimization of transgalactosylation activity of ?-galactosidase from Aspergillus oryzaeen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu